ExperimentExtractPlasmidDNA

ExperimentExtractPlasmidDNA[Samples]⟹Protocol

creates a Protocol object for isolating plasmid DNA from live cell or cell lysate Samples through lysing (if dealing with cells, rather than lysate), then neutralizing the pH of the solution to keep plasmid DNA soluble (through renaturing) and pelleting out insoluble cell components, followed by one or more rounds of optional purification techniques including precipitation (such as a cold ethanol or isopropanol wash), liquid-liquid extraction (such as phenol:chloroform extraction), solid phase extraction (such as spin columns), and magnetic bead separation (selectively binding plasmid DNA to magnetic beads while washing non-binding impurities from the mixture).

Details

Input

Output

Liquid-liquid Extraction Options

Lysis Options

Magnetic Bead Separation Options

{Transmogrify`Private`origwrappedWidth, Transmogrify`Private`origwrappedHeight} If[Ceiling[Transmogrify`Private`width$142870] > 1024, If[Transmogrify`Private`optDebug, Print[producing narrow 1024 image]]; Transmogrify`Private`defaultWindowSize = 1024; Transmogrify`Private`f = StringReplace[Files/ExperimentExtractPlasmidDNA.en/10.png, FileBaseName[Files/ExperimentExtractPlasmidDNA.en/10.png] :> FileBaseName[Files/ExperimentExtractPlasmidDNA.en/10.png]<>_1024]; Transmogrify`Private`boxToImageOptions = Flatten[{MaxImageSize -> 1024, Magnification -> Transmogrify`Private`magnification Transmogrify`Private`resolution, Transmogrify`Private`Resolution -> Transmogrify`Private`resolution, Sequence @@ Transmogrify`Private`options}]; Transmogrify`Private`btiWrappedImageReturn = BoxToImage[Transmogrify`Private`f, Sequence @@ Transmogrify`Private`boxToImageOptions]; If[Transmogrify`Private`optDebug, Print[btiWrappedImageReturn = <>ToString[Transmogrify`Private`btiWrappedImageReturn]]]; {Transmogrify`Private`origwrappedWidth, Transmogrify`Private`origwrappedHeight} = ToExpression[{Width, Height} /. Transmogrify`Private`btiWrappedImageReturn]; {Transmogrify`Private`wrappedWidth, Transmogrify`Private`wrappedHeight} = Ceiling[-------------------------------------------------------------------------------]; Return[XMLElement[img, {src -> (URL /. Transmogrify`Private`btiWrappedImageReturn), data-src -> (URL /. Transmogrify`Private`btiWrappedImageReturn), data-big -> ToString[Transmogrify`Private`defaultWidth]<> <>ToString[Transmogrify`Private`defaultHeight], data-small -> ToString[Transmogrify`Private`wrappedWidth]<> <>ToString[Transmogrify`Private`wrappedHeight], Sequence @@ {alt -> }}, {}]], If[Transmogrify`Private`optDebug, Print[narrow image branch, width <= 1024]]; Return[(XMLElement[img, {src -> (URL /. #1), height -> ToString[Transmogrify`Private`defaultHeight], width -> ToString[Transmogrify`Private`defaultWidth], Sequence @@ {alt -> }}, {}] & )[Transmogrify`Private`btiDefaultImageReturn]]] Transmogrify`Private`resolution

Neutralization Options

Organizational Information Options

Post Experiment Options

Precipitation Options

{Transmogrify`Private`origwrappedWidth, Transmogrify`Private`origwrappedHeight} If[Ceiling[Transmogrify`Private`width$143844] > 1024, If[Transmogrify`Private`optDebug, Print[producing narrow 1024 image]]; Transmogrify`Private`defaultWindowSize = 1024; Transmogrify`Private`f = StringReplace[Files/ExperimentExtractPlasmidDNA.en/18.png, FileBaseName[Files/ExperimentExtractPlasmidDNA.en/18.png] :> FileBaseName[Files/ExperimentExtractPlasmidDNA.en/18.png]<>_1024]; Transmogrify`Private`boxToImageOptions = Flatten[{MaxImageSize -> 1024, Magnification -> Transmogrify`Private`magnification Transmogrify`Private`resolution, Transmogrify`Private`Resolution -> Transmogrify`Private`resolution, Sequence @@ Transmogrify`Private`options}]; Transmogrify`Private`btiWrappedImageReturn = BoxToImage[Transmogrify`Private`f, Sequence @@ Transmogrify`Private`boxToImageOptions]; If[Transmogrify`Private`optDebug, Print[btiWrappedImageReturn = <>ToString[Transmogrify`Private`btiWrappedImageReturn]]]; {Transmogrify`Private`origwrappedWidth, Transmogrify`Private`origwrappedHeight} = ToExpression[{Width, Height} /. Transmogrify`Private`btiWrappedImageReturn]; {Transmogrify`Private`wrappedWidth, Transmogrify`Private`wrappedHeight} = Ceiling[-------------------------------------------------------------------------------]; Return[XMLElement[img, {src -> (URL /. Transmogrify`Private`btiWrappedImageReturn), data-src -> (URL /. Transmogrify`Private`btiWrappedImageReturn), data-big -> ToString[Transmogrify`Private`defaultWidth]<> <>ToString[Transmogrify`Private`defaultHeight], data-small -> ToString[Transmogrify`Private`wrappedWidth]<> <>ToString[Transmogrify`Private`wrappedHeight], Sequence @@ {alt -> }}, {}]], If[Transmogrify`Private`optDebug, Print[narrow image branch, width <= 1024]]; Return[(XMLElement[img, {src -> (URL /. #1), height -> ToString[Transmogrify`Private`defaultHeight], width -> ToString[Transmogrify`Private`defaultWidth], Sequence @@ {alt -> }}, {}] & )[Transmogrify`Private`btiDefaultImageReturn]]] Transmogrify`Private`resolution

Solid Phase Extraction Options

General Options

Examples

Basic Examples (8)

Living cells are lysed and neutralized to isolate plasmid DNA from the cell and then extracted plasmid DNA is purified:

Multiple cell samples and types can undergo plasmid DNA extraction in one experiment call:

Lysate samples can be neutralized and/or further purified using the rudimentary purification steps from plasmid DNA extraction:

Crude plasmid DNA samples can be further purified using the rudimentary purification steps from plasmid DNA extraction:

Crude samples can be purified with liquid-liquid extraction:

Crude samples can be purified with precipitation:

Crude samples can be purified with solid phase extraction:

Crude samples can be purified with magnetic bead separation:

Options (416)

AqueousSolvent (1)

If aqueous solvent is required for a separation, then AqueousSolvent will automatically be set to Model[Sample, "Milli-Q water"]:

AqueousSolventRatio (2)

If an AqueousSolventVolume is set, then the AqueousSolventRatio is calculated from the set AqueousSolventVolume and the sample volume (SampleVolume/AqueousSolventVolume):

If an AqueousSolvent is set and an AqueousSolventVolume is not set, then the AqueousSolventRatio is set to 5:

AqueousSolventVolume (2)

If an AqueousSolventRatio is set, then the AqueousSolventVolume is calculated from the ratio and the sample volume (SampleVolume/AqueousSolventRatio):

If an AqueousSolvent is set and an AqueousSolventRatio is not set, then the AqueousSolventVolume will be 20% of the sample volume:

CellType (2)

CellType is set to the value of the CellType field in the sample object (if specified in the object) and CultrueAdhesion is set to the value of the CultureAdhesion field of the sample object unless otherwise specified:

If the CellType field of the input sample is Unspecified, CellType automatically set to the majority cell type of the input sample based on its composition:

ClarifiedLysateContainer (1)

If ClarifyLysate is set to True, ClarifyLysateCentrifuge is automatically set to Model[Instrument, Centrifuge, "HiG4"], ClarifyLysateIntensity is automatically set to 5700 RPM, ClarifyLysateTime is automatically set to 10 minutes, ClarifiedLysateVolume is set automatically set to 90% of the volume of the lysate, PostClarificationPelletLabel will be automatically generated, PostClarificationPelletStorageCondition will be automatically set to Disposal, ClarifiedLysateContainer will be automatically selected to accommodate the volume of the clarified lysate, and ClarifiedLysateContainerLabel will be automatically generated unless otherwise specified:

ClarifiedLysateContainerLabel (1)

ClarifiedLysateVolume (1)

ClarifyLysate (2)

If any clarification options are set (ClarifyLysateCentrifuge, ClarifyLysateIntensity, ClarifyLysateTime, ClarifiedLysateVolume, ClarifiedLysateContainer, and PostClarificationPelletStorageCondition), then ClarifyLysate is set to True:

If no clarification options are set (ClarifyLysateCentrifuge, ClarifyLysateIntensity, ClarifyLysateTime, ClarifiedLysateVolume, ClarifiedLysateContainer, and PostClarificationPelletStorageCondition), then ClarifyLysate is set to False:

ClarifyLysateCentrifuge (1)

ClarifyLysateIntensity (1)

ClarifyLysateTime (1)

CollectSecondarySolidPhaseExtractionWashFlowthrough (1)

If SolidPhaseExtraction is included in Purification, then SolidPhaseExtractionStrategy is automatically set to Positive, SolidPhaseExtractionTechnique is set to Pressure, SolidPhaseExtractionCartridgeStorageCondition is automatically set to Disposal, SolidPhaseExtractionLoadingTime is set to 1 Minute, SolidPhaseExtractionCartridge is automatically set to Model[Container, Plate, Filter, "NucleoSpin Plasmid Binding Plate"], SolidPhaseExtractionWashSolution is automatically set to Model[Sample, "Macherey-Nagel Wash Buffer A4"], SolidPhaseExtractionWashTime is automatically set to 1 Minute, CollectSolidPhaseExtractionWashFlowthrough is automatically set to False, SecondarySolidPhaseExtractionWashSolution is automatically set to Model[Sample, "Macherey-Nagel Wash Buffer A4"], SecondarySolidPhaseExtractionWashTime is automatically set to 1 Minute, CollectSecondarySolidPhaseExtractionWashFlowthrough is automatically set to False, TertiarySolidPhaseExtractionWashSolution is automatically set to Model[Sample, "Macherey-Nagel Wash Buffer A4"], TertiarySolidPhaseExtractionWashTime is automatically set to 1 Minute, CollectTertiarySolidPhaseExtractionWashFlowthrough is automatically set to False, SolidPhaseExtractionElutionSolution is automatically set to Model[Sample, "Macherey-Nagel Elution Buffer AE"], SolidPhaseExtractionElutionTime is automatically set to 1 Minute unless otherwise specified:

CollectSolidPhaseExtractionLoadingFlowthrough (2)

If SolidPhaseExtractionStrategy is set to Negative, then CollectSolidPhaseExtractionLoadingFlowthrough is automatically set to True:

If SolidPhaseExtractionStrategy is set to Positive, then CollectSolidPhaseExtractionLoadingFlowthrough is automatically set to False:

CollectSolidPhaseExtractionWashFlowthrough (1)

CollectTertiarySolidPhaseExtractionWashFlowthrough (1)

ContainerOut (1)

ContainerOut is automatically set to the container that the sample was in for the last step of the extraction unless otherwise specified:

CultureAdhesion (1)

Demulsifier (3)

If DemulsifierAdditions are specified, then Demulsifier will automatically be set to the specified demulsifier in DemulsifierAdditions:

If a DemulsifierAmount is specified, then Demulsifier will automatically be set to Model[Sample, StockSolution, "5M Sodium Chloride"]:

If neither DemulsifierAdditions nor DemulsifierAmount are set, then a Demulsifier is automatically set to None:

DemulsifierAdditions (4)

If a Demulsifier is not specified, then the DemulsifierAdditions are set to None for each round:

If there will only be one round of liquid-liquid extraction and a Demulsifier is specified, then the Demulsifier will be added for that one extraction round in DemulsifierAdditions:

If there will be multiple rounds of liquid-liquid extraction, a Demulsifier is specified, and the organic phase will be used in subsequent extraction rounds, then the Demulsifier will be added for each extraction round in DemulsifierAdditions (since the demulsifier will likely be removed with the aqueous phase each round):

If there will be multiple rounds of liquid-liquid extraction, a Demulsifier is specified, and the aqueous phase will be used in subsequent extraction rounds, then the Demulsifier will only be added for the first extraction round in DemulsifierAdditions (since the demulsifier is usually a salt solution which stays in the aqueous phase):

DemulsifierAmount (1)

If Demulsifier and/or DemulsifierAdditions are specified, then the DemulsifierAmount is set to 10% of the sample volume:

Dissociate (3)

If CultureAdhesion is Adherent and LysisAliquot is True, then Dissociate is set to True:

If CultureAdhesion is Suspension, then Dissociate is set to False:

If CultureAdhesion is Adherent but LysisAliquot is False, then Dissociate is set to False:

ElutionMagnetizationTime (1)

If MagneticBeadSeparationElution is set to True, MagneticBeadSeparationElutionCollectionContainerLabel is generated, MagneticBeadSeparationElutionSolution is set to Model[Sample, "Zyppy Elution Buffer"], MagneticBeadSeparationElutionMix is set to True, ElutionMagnetizationTime is set to 5 minutes, MagneticBeadSeparationElutionCollectionContainer is set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationElutionCollectionStorageCondition is set to Refrigerator, and NumberOfMagneticBeadSeparationElutions is set to 1 unless otherwise specified:

EquilibrationMagnetizationTime (1)

If MagneticBeadSeparationEquilibration is set to True, MagneticBeadSeparationEquilibrationSolution is automatically set to Model[Sample,"Milli-Q water"], MagneticBeadSeparationEquilibrationMix is automatically set to True, EquilibrationMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationEquilibrationCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationEquilibrationCollectionStorageCondition is automatically set to Refrigerator, MagneticBeadSeparationEquilibrationAirDry is automatically set to False, and MagneticBeadSeparationEquilibrationCollectionContainerLabel is automatically generated unless otherwise specified:

ExtractedPlasmidDNAContainerLabel (1)

ExtractedPlasmidDNALabel and ExtractedPlasmidDNAContainerLabel are automatically generated if they are not otherwise specified:

ExtractedPlasmidDNALabel (1)

ExtractedPlasmidDNALabel and ExtractedPlasmidDNAContainerLabel are automatically generated if they are not otherwise specified:

ImpurityLayerContainerLabel (1)

If LiquidLiquidExtraction is included in Purification, LiquidLiquidExtractionTemperature is automatically set to Ambient, NumberOfLiquidLiquidExtractions is automatically set to 3, LiquidLiquidExtractionSettlingTime is set to 1 minute, ImpurityLayerStorageCondition is set to Disposal for each extraction round, ImpurityLayerContainerOutWell is automatically set to the first empty well of the ImpurityLayerContainerOut, ImpurityLayerLabel is automatically generated, and ImpurityLayerContainerLabel is automatically generated unless otherwise specified:

ImpurityLayerContainerOut (2)

If LiquidLiquidExtractionTargetPhase is set to Aqueous and LiquidLiquidExtractionTechnique is set to PhaseSeparator, ImpurityLayerContainerOut is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"]:

If LiquidLiquidExtractionTargetPhase is not set to Aqueous or LiquidLiquidExtractionTechnique is not set to PhaseSeparator, ImpurityLayerContainerOut is automatically determined by the PreferredContainer function:

ImpurityLayerContainerOutWell (1)

ImpurityLayerLabel (1)

ImpurityLayerStorageCondition (1)

IncludeLiquidBoundary (1)

If LiquidLiquidExtractionTechnique is set to Pipette, then IncludeLiquidBoundary is automatically set to False for each extraction round and LiquidLiquidExtractionTransferLayer will be set to Top for each extraction round unless otherwise specified:

LiquidBoundaryVolume (2)

If LiquidLiquidExtractionTechnique is set to Pipette and LiquidLiquidExtractionTransferLayer is set to Top for the first extraction round, then LiquidBoundaryVolume will be set to a 5 millimeter tall cross-section of the sample container at the location of the layer boundary for each extraction round:

If LiquidLiquidExtractionTechnique is set to Pipette and LiquidLiquidExtractionTransferLayer is set to Bottom for the first extraction round, then LiquidBoundaryVolume will be set to a 5 millimeter tall cross-section of the bottom of the sample container for each extraction round:

LiquidLiquidExtractionCentrifuge (3)

If any centrifuging options (LiquidLiquidExtractionCentrifugeInstrument, LiquidLiquidExtractionCentrifugeTime, and LiquidLiquidExtractionCentrifugeIntensity) are specified, then LiquidLiquidExtractionCentrifuge will automatically be set to True:

If LiquidLiquidExtractionTechnique is set to Pipette and the sample is in a centrifuge-compatible container, then LiquidLiquidExtractionCentrifuge will automatically be set to True:

If no centrifuging options (LiquidLiquidExtractionCentrifugeInstrument, LiquidLiquidExtractionCentrifugeTime, and LiquidLiquidExtractionCentrifugeIntensity) are set, LiquidLiquidExtractionTechnique is set to PhaseSeparator, or the sample is not in a centrifuge-compatible container, then LiquidLiquidExtractionCentrifuge will automatically be set to False:

LiquidLiquidExtractionCentrifugeInstrument (1)

If LiquidLiquidExtractionCentrifuge is set to True, then LiquidLiquidExtractionCentrifugeInstrument will be set to the integrated centrifuge model on the chosen robotic instrument and LiquidLiquidExtractionCentrifugeTime is automatically set to 2 minutes unless otherwise specified:

LiquidLiquidExtractionCentrifugeIntensity (1)

If the liquid-liquid extraction solution will be centrifuged and the MaxIntensity of the centrifuge model is less than the MaxCentrifugationForce of the plate model (or the MaxCentrifugationForce of the plate model does not exist), the liquid-liquid extraction solution will be centrifuged at the MaxIntensity of the centrifuge model:

LiquidLiquidExtractionCentrifugeTime (1)

LiquidLiquidExtractionContainer (3)

If the sample is in a non-centrifuge-compatible container and LiquidLiquidExtractionCentrifuge is set to True, then LiquidLiquidExtractionContainer will be automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"]:

If heating or cooling is specified, then LiquidLiquidExtractionContainer will be automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"] (since the robotic heater/cooler units are only compatible with Plate format containers):

If LiquidLiquidExtractionCentrifuge is set to True and the extraction will take place at ambient temperature, then the extraction container will be selected using the PreferredContainer function (to find a robotic-compatible container that will hold the sample):

LiquidLiquidExtractionContainerWell (1)

If a LiquidLiquidExtractionContainer is specified, then LiquidLiquidExtractionContainerWell will automatically be set to the first available well in the container:

LiquidLiquidExtractionDevice (1)

If LiquidLiquidExtractionTechnique is set to PhaseSeparator, then LiquidLiquidExtractionDevice is automatically set to Model[Container,Plate,PhaseSeparator,"Semi-Transparent Plastic 96 Fixed Well Plate with Phase Separator Frits"]:

LiquidLiquidExtractionMixRate (1)

If LiquidLiquidExtractionMixType is set to Shake, then LiquidLiquidExtractionMixTime is automatically set to 30 seconds and LiquidLiquidExtractionMixRate is set to 300 RPM unless otherwise specified:

LiquidLiquidExtractionMixTime (1)

If LiquidLiquidExtractionMixType is set to Shake, then LiquidLiquidExtractionMixTime is automatically set to 30 seconds and LiquidLiquidExtractionMixRate is set to 300 RPM unless otherwise specified:

LiquidLiquidExtractionMixType (2)

If LiquidLiquidExtractionMixTime or LiquidLiquidExtractionMixRate is specified, then LiquidLiquidExtractionMixType will automatically be set to Shake:

If mixing options (LiquidLiquidExtractionMixTime and LiquidLiquidExtractionMixRate) are not specified, then LiquidLiquidExtractionMixType will automatically be set to Pipette:

LiquidLiquidExtractionMixVolume (2)

If half of the liquid-liquid extraction mixture (the sample volume plus the entire volume of the added aqueous solvent, organic solvent, and demulsifier if specified) for each extraction is less than 970 microliters, then LiquidLiquidExtractionMixVolume will be automatically set to half of the liquid-liquid extraction mixture:

If half of the liquid-liquid extraction mixture (the sample volume plus the entire volume of the added aqueous solvent, organic solvent, and demulsifier if specified) for each extraction is greater than 970 microliters, then LiquidLiquidExtractionMixVolume will automatically be set to 970 microliters (the maximum amount that can be mixed via pipette on the liquid handling robot):

LiquidLiquidExtractionSelectionStrategy (4)

If NumberOfLiquidLiquidExtractions is set to one, then LiquidLiquidExtractionSelectionStrategy is not specified (since a selection strategy is only required for multiple extraction rounds):

If the phase of the solvent being added for each liquid-liquid extraction round matches the target phase, then LiquidLiquidExtractionSelectionStrategy is automatically set to Positive:

If the phase of the solvent being added for each liquid-liquid extraction round does not match the target phase, then LiquidLiquidExtractionSelectionStrategy is automatically set to Negative:

If LiquidLiquidExtractionTargetPhase and AqueousSolvent/OrganicSovlent are not set, then LiquidLiquidExtractionSelectionStrategy is automatically set to Positive:

LiquidLiquidExtractionSettlingTime (1)

LiquidLiquidExtractionSolventAdditions (3)

If the starting sample of any extraction round is in an organic or unknown phase, then an aqueous solvent is added to that sample:

If the starting sample of any extraction round is in an aqueous phase, then organic solvent is added to that sample:

If the starting sample of an extraction is biphasic, then no solvent is added for the first extraction:

LiquidLiquidExtractionTargetLayer (5)

The target layer for each extraction round is calculated from the density of the sample's aqueous and organic layers (if present in the sample), the density of the AqueousSolvent and OrganicSolvent options (if specified), and the target phase:

If the target phase is aqueous and the aqueous phase is less dense than the organic phase, then the target layer will be the top layer:

If the target phase is aqueous and the aqueous phase is denser than the organic phase, then the target layer will be the bottom layer:

If the target phase is organic and the aqueous phase is less dense than the organic phase, then the target layer will be the bottom layer:

If the target phase is organic and the aqueous phase is denser than the organic phase, then the target layer will be the top layer:

LiquidLiquidExtractionTargetPhase (1)

The target phase is selected based on the phase that the plasmid DNA is more likely to be found in based on the PredictDestinationPhase[...] function:

LiquidLiquidExtractionTechnique (2)

If pipetting-specific options (IncludeLiquidBoundary or LiquidBoundaryVolume) are set, then LiquidLiquidExtractionTechnique is automatically set to Pipette:

If pipetting-specific options (IncludeLiquidBoundary or LiquidBoundaryVolume) are not set, then LiquidLiquidExtractionTechnique is automatically set to PhaseSeparator:

LiquidLiquidExtractionTemperature (1)

LiquidLiquidExtractionTransferLayer (1)

LoadingMagnetizationTime (1)

By default, MagneticBeadSeparationSelectionStrategy is set to Positive, MagneticBeadSeparationMode is set to NormalPhase, MagneticBeadCollectionStorageCondition is automatically set to Disposal, MagnetizationRack is automatically set to Model[Item, MagnetizationRack, "Alpaqua 96S Super Magnet 96-well Plate Rack"], MagneticBeadSeparationLoadingMix is automatically set to True, LoadingMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationLoadingCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationLoadingCollectionStorageCondition is automatically set to Refrigerator, and MagneticBeadSeparationLoadingAirDry is automatically set to False unless otherwise specified:

Lyse (2)

If the input is living cells, they will be lysed before neutralization and purification:

If the input is lysate or crude plasmid solution, it will not be lysed (since it is already lysed or does not require lysing):

LysisAliquot (2)

If LysisAliquotAmount, LysisAliquotContainer, TargetCellCount, or TargetCellConcentration are specified, LysisAliquot is set to True:

If no aliquotting options are set (LysisAliquotAmount, LysisAliquotContainer, TargetCellCount, or TargetCellConcentration), LysisAliquot is set to False:

LysisAliquotAmount (4)

If LysisAliquot is set to True and TargetCellCount is set, LysisAliquotAmount will be set to attain the target cell count:

If LysisAliquotContainer is specified but TargetCellCount is not specified, LysisAliquotAmount will be set to All if the input sample is less than half of the LysisAliquotContiner's max volume:

If LysisAliquotContainer is specified but TargetCellCount is not specified, LysisAliquotAmount will be set to half of the LysisAliquotContiner's max volume if the input sample volume is greater than that value:

If LysisAliquotContainer and TargetCellCount are not specified, LysisAliquotAmount will be set to 25% of the input sample volume:

LysisAliquotContainer (1)

If LysisAliquot is True, LysisAliquotContainer will be assigned by PackContainers and LysisAliquotContainerLabel will be automatically generated, :

LysisAliquotContainerLabel (1)

If LysisAliquot is True, LysisAliquotContainer will be assigned by PackContainers and LysisAliquotContainerLabel will be automatically generated, :

LysisIncubationInstrument (1)

If Lyse is set to True, LysisSolution is automatically set to Model[Sample, StockSolution, "Alkaline lysis buffer for Plasmid DNA extraction"], LysisTime is automatically set to 15 minutes, LysisTemperature is automatically set to Ambient, and LysisIncubationInstrument is automatically set to an integrated instrument compatible with the specified LysisTemperature, unless otherwise specified:

LysisMixInstrument (1)

If LysisMixType is set to Shake, LysisMixRate is automatically set to 200 RPM, LysisMixTime is automatically set to 1 minute, and LysisMixInstrument is automatically set to an available shaking device compatible with the specified LysisMixRate and LysisMixTemperature unless otherwise specified:

LysisMixRate (1)

LysisMixTemperature (1)

If LysisMixType is set to either Pipette or Shake, LysisMixTemperature is automatically set to Ambient unless otherwise specified:

LysisMixTime (1)

LysisMixType (4)

If LysisMixVolume or NumberOfLysisMixes are specified, LysisMixType is automatically set to Pipette:

If LysisMixRate, LysisMixTime, or LysisMixInstrument are specified, LysisMixType is automatically set to Shake:

If no mixing options are set and the sample will be mixed in a plate, LysisMixType is automatically set to Shake:

If no mixing options are set and the sample will be mixed in a tube, LysisMixType is automatically set to Pipette:

LysisMixVolume (2)

If LysisMixType is set to Pipette and 50% of the total solution volume is less than 970 microliters, LysisMixVolume is automatically set to 50% of the total solution volume:

If LysisMixType is set to Pipette and 50% of the total solution volume is greater than 970 microliters, LysisMixVolume is automatically set to 970 microliters:

LysisSolution (1)

LysisSolutionVolume (3)

If LysisAliquot is False, LysisSolutionVolume is automatically set to 50% of the unoccupied volume of the sample's container divided by the NumberOfLysisSteps:

If LysisAliquotContainer is set, LysisSolutionVolume is automatically set to 50% of the unoccupied volume of the LysisAliquotContainer (prior to addition of any lysis solutions) divided by the NumberOfLysisSteps:

If LysisAliquot is True and LysisAliquotContainer is not set, LysisSolutionVolume is automatically set to nine times the LysisAliquotAmount divided by the NumberOfLysisSteps:

LysisTemperature (1)

LysisTime (1)

MagneticBeadAffinityLabel (1)

If MagneticBeadSeparationMode is set to Affinity,MagneticBeadSeparationAnalyteAffinityLabel is automatically set to the first item in Analytes of the sample, MagneticBeadAffinityLabel is automatically set to the first item in the Targets field of the target molecule object, and MagneticBeads is automatically set to the first found magnetic beads model with the affinity label of MagneticBeadAffinityLabel (or Model[Sample, "Mag-Bind Particles CNR (Mag-Bind Viral DNA/RNA Kit)" if there are no beads with the affinity label of MagneticBeadAffinityLabel)]:

MagneticBeadCollectionStorageCondition (1)

MagneticBeads (2)

If MagneticBeadSeparationMode is not set to Affinity, MagneticBeads is automatically set based on the MagneticBeadSeparationMode and the target of the extraction:

MagneticBeadSeparationAnalyteAffinityLabel (1)

MagneticBeadSeparationElution (3)

If any magnetic bead separation elution options are set (MagneticBeadSeparationElutionSolution, MagneticBeadSeparationElutionSolutionVolume, MagneticBeadSeparationElutionMix, etc.) or MagneticBeadSeparationSelectionStrategy is set to Positive, MagneticBeadSeparationElution is automatically set to True:

If MagneticBeadSeparationSelectionStrategy is Positive, MagneticBeadSeparationElution is automatically set to True:

If no magnetic bead separation elution options are set (MagneticBeadSeparationElutionSolution, MagneticBeadSeparationElutionSolutionVolume, MagneticBeadSeparationElutionMix, etc.) and MagneticBeadSeparationSelectionStrategy is Negative, MagneticBeadSeparationElution is automatically set to False:

MagneticBeadSeparationElutionAspirationVolume (1)

If MagneticBeadSeparationElution is set to True, MagneticBeadSeparationElutionSolutionVolume is automatically set 1/10 of the MagneticBeadSeparationSampleVolume and MagneticBeadSeparationElutionAspirationVolume is automatically set the same as the MagneticBeadSeparationElutionSolutionVolume:

MagneticBeadSeparationElutionCollectionContainer (1)

MagneticBeadSeparationElutionCollectionContainerLabel (1)

MagneticBeadSeparationElutionCollectionStorageCondition (1)

MagneticBeadSeparationElutionMix (1)

MagneticBeadSeparationElutionMixRate (1)

If MagneticBeadSeparationElutionMixType is set to Shake, MagneticBeadSeparationElutionMixTime is automatically set to 5 minutes and MagneticBeadSeparationElutionMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationElutionMixTemperature (1)

If MagneticBeadSeparationElutionMix is set to True, MagneticBeadSeparationElutionMixVolume is automatically set to Ambient:

MagneticBeadSeparationElutionMixTime (1)

MagneticBeadSeparationElutionMixTipMaterial (1)

If MagneticBeadSeparationElutionMixType is set to Pipette, NumberOfMagneticBeadSeparationElutionMixes is automatically set to 10, MagneticBeadSeparationElutionMixTipType is automatically set to WideBore, and MagneticBeadSeparationElutionMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationElutionMixTipType (1)

MagneticBeadSeparationElutionMixType (3)

If MagneticBeadSeparationElutionMixRate or MagneticBeadSeparationElutionMixTime are set, MagneticBeadSeparationElutionMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationElutionMixes or MagneticBeadSeparationElutionMixVolume are set, MagneticBeadSeparationElutionMixType is automatically set to Pipette:

If no magnetic bead separation elution mix options are set, MagneticBeadSeparationElutionMixType is automatically set to Pipette:

MagneticBeadSeparationElutionMixVolume (2)

If MagneticBeadSeparationElutionMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationElutionSolutionVolume and MagneticBeadVolume is less than 970 microliters, MagneticBeadSeparationElutionMixVolume is automatically set to 1/2 of theMagneticBeadSeparationElutionSolutionVolume:

If MagneticBeadSeparationElutionMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationElutionSolutionVolume and MagneticBeadVolume is greater than 970 microliters, MagneticBeadSeparationElutionMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationElutionSolution (1)

MagneticBeadSeparationElutionSolutionVolume (1)

MagneticBeadSeparationEquilibration (2)

If any magnetic bead separation equilibration options are set (MagneticBeadSeparationEquilibrationSolution, MagneticBeadSeparationEquilibrationSolutionVolume, MagneticBeadSeparationEquilibrationMix, etc.), MagneticBeadSeparationEquilibration is automatically set to True:

If no magnetic bead separation equilibration options are set (MagneticBeadSeparationEquilibrationSolution, MagneticBeadSeparationEquilibrationSolutionVolume, MagneticBeadSeparationEquilibrationMix, etc.), MagneticBeadSeparationEquilibration is automatically set to False:

MagneticBeadSeparationEquilibrationAirDry (1)

MagneticBeadSeparationEquilibrationAirDryTime (1)

If MagneticBeadSeparationEquilibrationAirDry is set to True, MagneticBeadSeparationEquilibrationAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationEquilibrationAspirationVolume (1)

If MagneticBeadSeparationEquilibration is set to True, MagneticBeadSeparationEquilibrationAspirationVolume is automatically set the same as the MagneticBeadSeparationEquilibrationSolutionVolume:

MagneticBeadSeparationEquilibrationCollectionContainer (1)

MagneticBeadSeparationEquilibrationCollectionContainerLabel (1)

MagneticBeadSeparationEquilibrationCollectionStorageCondition (1)

MagneticBeadSeparationEquilibrationMix (1)

MagneticBeadSeparationEquilibrationMixRate (1)

If MagneticBeadSeparationEquilibrationMixType is set to Shake, MagneticBeadSeparationEquilibrationMixTime is automatically set to 5 minutes and MagneticBeadSeparationEquilibrationMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationEquilibrationMixTemperature (1)

If MagneticBeadSeparationEquilibrationMix is set to True, MagneticBeadSeparationEquilibrationMixVolume is automatically set to Ambient:

MagneticBeadSeparationEquilibrationMixTime (1)

MagneticBeadSeparationEquilibrationMixTipMaterial (1)

If MagneticBeadSeparationEquilibrationMixType is set to Pipette, NumberOfMagneticBeadSeparationEquilibrationMixes is automatically set to 10, MagneticBeadSeparationEquilibrationMixTipType is automatically set to WideBore, and MagneticBeadSeparationEquilibrationMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationEquilibrationMixTipType (1)

MagneticBeadSeparationEquilibrationMixType (3)

If MagneticBeadSeparationEquilibrationMixRate or MagneticBeadSeparationEquilibrationMixTime are set, MagneticBeadSeparationEquilibrationMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationEquilibrationMixes or MagneticBeadSeparationEquilibrationMixVolume are set, MagneticBeadSeparationEquilibrationMixType is automatically set to Pipette:

If no magnetic bead separation equilibration mix options are set, MagneticBeadSeparationEquilibrationMixType is automatically set to Pipette:

MagneticBeadSeparationEquilibrationMixVolume (2)

If MagneticBeadSeparationEquilibrationMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationEquilibrationSolution and magnetic beads volume is less than 970 microliters, MagneticBeadSeparationEquilibrationMixVolume is automatically set to 1/2 of the combined MagneticBeadSeparationEquilibrationSolution and magnetic beads volume:

If MagneticBeadSeparationEquilibrationMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationEquilibrationSolution and magnetic beads volume is greater than 970 microliters, MagneticBeadSeparationEquilibrationMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationEquilibrationSolution (1)

MagneticBeadSeparationEquilibrationSolutionVolume (1)

If MagneticBeadSeparationEquilibration is set to True, MagneticBeadSeparationEquilibrationSolution is automatically set to the same value as the MagneticBeadSeparationPreWashSolutionVolume:

MagneticBeadSeparationLoadingAirDry (1)

MagneticBeadSeparationLoadingAirDryTime (1)

If MagneticBeadSeparationLoadingAirDry is set to True, MagneticBeadSeparationLoadingAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationLoadingAspirationVolume (1)

MagneticBeadSeparationLoadingAspirationVolume is automatically set the same as the MagneticBeadSeparationSampleVolume:

MagneticBeadSeparationLoadingCollectionContainer (1)

MagneticBeadSeparationLoadingCollectionContainerLabel (1)

MagneticBeadSeparationLoadingCollectionContainerLabel is automatically generated unless otherwise specified:

MagneticBeadSeparationLoadingCollectionStorageCondition (1)

MagneticBeadSeparationLoadingMixRate (1)

If MagneticBeadSeparationLoadingMixType is set to Shake, MagneticBeadSeparationLoadingMixTime is automatically set to 5 minutes and MagneticBeadSeparationLoadingMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationLoadingMixTemperature (1)

If MagneticBeadSeparationLoadingMix is set to True, MagneticBeadSeparationLoadingMixVolume is automatically set to Ambient:

MagneticBeadSeparationLoadingMixTime (1)

MagneticBeadSeparationLoadingMixTipMaterial (1)

If MagneticBeadSeparationLoadingMixType is set to Pipette, NumberOfMagneticBeadSeparationLoadingMixes is automatically set to 10, MagneticBeadSeparationLoadingMixTipType is automatically set to WideBore, and MagneticBeadSeparationLoadingMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationLoadingMixTipType (1)

MagneticBeadSeparationLoadingMixType (3)

If MagneticBeadSeparationLoadingMixRate or MagneticBeadSeparationLoadingMixTime are set, MagneticBeadSeparationLoadingMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationLoadingMixes or MagneticBeadSeparationLoadingMixVolume are set, MagneticBeadSeparationLoadingMixType is automatically set to Pipette:

If no magnetic bead separation equilibration mix options are set, MagneticBeadSeparationLoadingMixType is automatically set to Pipette:

MagneticBeadSeparationLoadingMixVolume (2)

If MagneticBeadSeparationLoadingMixType is set to Pipette and 50% of the MagneticBeadSeparationSampleVolume is less than 970 microliters, MagneticBeadSeparationLoadingMixVolume is automatically set to 50% of the MagneticBeadSeparationSampleVolume:

If MagneticBeadSeparationLoadingMixType is set to Pipette and set to 50% of the MagneticBeadSeparationSampleVolume is greater than 970 microliters, MagneticBeadSeparationLoadingMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationMode (1)

MagneticBeadSeparationPreWash (2)

If other magnetic bead separation prewash options are set, then MagneticBeadSeparationPreWash is automatically set to True:

If no magnetic bead separation prewash options are set, then MagneticBeadSeparationPreWash is automatically set to False:

MagneticBeadSeparationPreWashAirDry (1)

If MagneticBeadSeparationPreWash is set to True, MagneticBeadSeparationPreWashSolutionVolume is automatically set to the sample volume, MagneticBeadSeparationPreWashMix is automatically set to True, PreWashMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationPreWashCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationPreWashCollectionStorageCondition is automatically set to Refrigerator, NumberOfMagneticBeadSeparationPreWashes is automatically set to 1, MagneticBeadSeparationPreWashAirDry is automatically set to False, and MagneticBeadSeparationPreWashCollectionContainerLabel is automatically generated unless otherwise specified:

MagneticBeadSeparationPreWashAirDryTime (1)

If MagneticBeadSeparationPreWashAirDry is True, MagneticBeadSeparationPreWashAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationPreWashAspirationVolume (1)

If MagneticBeadSeparationPreWash is True, MagneticBeadSeparationPreWashAspirationVolume is automatically set to the MagneticBeadSeparationPreWashSolutionVolume unless otherwise specified:

MagneticBeadSeparationPreWashCollectionContainer (1)

MagneticBeadSeparationPreWashCollectionContainerLabel (1)

MagneticBeadSeparationPreWashCollectionStorageCondition (1)

MagneticBeadSeparationPreWashMix (1)

MagneticBeadSeparationPreWashMixRate (1)

If MagneticBeadSeparationPreWashMixType is set to Shake, MagneticBeadSeparationPreWashMixTime is automatically set to 5 Minute and MagneticBeadSeparationPreWashMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationPreWashMixTemperature (1)

If MagneticBeadSeparationPreWashMix is True, MagneticBeadSeparationPreWashMixTemperature is automatically set to Ambient unless otherwise specified:

MagneticBeadSeparationPreWashMixTime (1)

MagneticBeadSeparationPreWashMixTipMaterial (1)

If MagneticBeadSeparationPreWashMixType is set to Pipette, NumberOfMagneticBeadSeparationPreWashMixes is automatically set to 10, MagneticBeadSeparationPreWashMixTipType is automatically set to WideBore, and MagneticBeadSeparationPreWashMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationPreWashMixTipType (1)

MagneticBeadSeparationPreWashMixType (3)

If MagneticBeadSeparationPreWashMix is set to True and shaking options are set, MagneticBeadSeparationPreWashMixType is automatically set to Shake:

If MagneticBeadSeparationPreWashMix is set to True and pipetting options are set, MagneticBeadSeparationPreWashMixType is automatically set to Pipette:

If MagneticBeadSeparationPreWashMix is set to True and no other mix options are set, MagneticBeadSeparationPreWashMixType is automatically set to Pipette:

MagneticBeadSeparationPreWashMixVolume (2)

If MagneticBeadSeparationPreWashMixType is set to Pipette and 50% of the combined MagneticBeadSeparationPreWashSolutionVolume and magnetic beads volume is less than the MaxRoboticSingleTransferVolume (0.970 mL), MagneticBeadSeparationPreWashMixVolume is automatically set to 50% of the combined MagneticBeadSeparationPreWashSolutionVolume and magnetic beads volume:

If MagneticBeadSeparationPreWashMixType is set to Pipette and 50% of the combined MagneticBeadSeparationPreWashSolutionVolume and magnetic beads volume is greater than the MaxRoboticSingleTransferVolume (0.970 mL), MagneticBeadSeparationPreWashMixVolume is automatically set to the MaxRoboticSingleTransferVolume (0.970 mL):

MagneticBeadSeparationPreWashSolution (1)

If MagneticBeadSeparationPreWash is set to True, MagneticBeadSeparationPreWashSolution is automatically set to match the MagneticBeadSeparationElutionSolution:

MagneticBeadSeparationPreWashSolutionVolume (1)

MagneticBeadSeparationQuaternaryWash (2)

If any magnetic bead separation quaternary wash options are set (MagneticBeadSeparationQuaternaryWashSolution, MagneticBeadSeparationQuaternaryWashSolutionVolume, MagneticBeadSeparationQuaternaryWashMix, etc.), MagneticBeadSeparationQuaternaryWash is automatically set to True:

If no magnetic bead separation quaternary wash options are set (MagneticBeadSeparationQuaternaryWashSolution, MagneticBeadSeparationQuaternaryWashSolutionVolume, MagneticBeadSeparationQuaternaryWashMix, etc.), MagneticBeadSeparationQuaternaryWash is automatically set to False:

MagneticBeadSeparationQuaternaryWashAirDry (1)

If MagneticBeadSeparationQuaternaryWash is set to True, MagneticBeadSeparationQuaternaryWashSolution is automatically set to Model[Sample,"Zyppy Wash Buffer"], MagneticBeadSeparationQuaternaryWashMix is automatically set to True, QuaternaryWashMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationQuaternaryWashCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationQuaternaryWashCollectionStorageCondition is automatically set to Refrigerator, NumberOfMagneticBeadSeparationQuaternaryWashes is automatically set to 1, MagneticBeadSeparationQuaternaryWashAirDry is automatically set to False, and MagneticBeadSeparationQuaternaryWashCollectionContainerLabel is automatically generated unless otherwise specified:

MagneticBeadSeparationQuaternaryWashAirDryTime (1)

If MagneticBeadSeparationQuaternaryWashAirDry is set to True, MagneticBeadSeparationQuaternaryWashAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationQuaternaryWashAspirationVolume (1)

If MagneticBeadSeparationQuaternaryWash is set to True, MagneticBeadSeparationQuaternaryWashAspirationVolume is automatically set the same as the MagneticBeadSeparationQuaternaryWashSolutionVolume:

MagneticBeadSeparationQuaternaryWashCollectionContainer (1)

MagneticBeadSeparationQuaternaryWashCollectionContainerLabel (1)

MagneticBeadSeparationQuaternaryWashCollectionStorageCondition (1)

MagneticBeadSeparationQuaternaryWashMix (1)

MagneticBeadSeparationQuaternaryWashMixRate (1)

If MagneticBeadSeparationQuaternaryWashMixType is set to Shake, MagneticBeadSeparationQuaternaryWashMixTime is automatically set to 5 minutes and MagneticBeadSeparationQuaternaryWashMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationQuaternaryWashMixTemperature (1)

If MagneticBeadSeparationQuaternaryWashMix is set to True, MagneticBeadSeparationQuaternaryWashMixVolume is automatically set to Ambient:

MagneticBeadSeparationQuaternaryWashMixTime (1)

MagneticBeadSeparationQuaternaryWashMixTipMaterial (1)

If MagneticBeadSeparationQuaternaryWashMixType is set to Pipette, NumberOfMagneticBeadSeparationQuaternaryWashMixes is automatically set to 10, MagneticBeadSeparationQuaternaryWashMixTipType is automatically set to WideBore, and MagneticBeadSeparationQuaternaryWashMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationQuaternaryWashMixTipType (1)

MagneticBeadSeparationQuaternaryWashMixType (3)

If MagneticBeadSeparationQuaternaryWashMixRate or MagneticBeadSeparationQuaternaryWashMixTime are set, MagneticBeadSeparationQuaternaryWashMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationQuaternaryWashMixes or MagneticBeadSeparationQuaternaryWashMixVolume are set, MagneticBeadSeparationQuaternaryWashMixType is automatically set to Pipette:

If no magnetic bead separation quaternary wash mix options are set, MagneticBeadSeparationQuaternaryWashMixType is automatically set to Pipette:

MagneticBeadSeparationQuaternaryWashMixVolume (2)

If MagneticBeadSeparationQuaternaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationQuaternaryWashSolution and magnetic beads volume is less than 970 microliters, MagneticBeadSeparationQuaternaryWashMixVolume is automatically set to 1/2 of the combined MagneticBeadSeparationQuaternaryWashSolution and magnetic beads volume:

If MagneticBeadSeparationQuaternaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationQuaternaryWashSolution and magnetic beads volume is greater than 970 microliters, MagneticBeadSeparationQuaternaryWashMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationQuaternaryWashSolution (1)

MagneticBeadSeparationQuaternaryWashSolutionVolume (2)

If MagneticBeadSeparationQuaternaryWash and MagneticBeadSeparationPreWash are set to True, MagneticBeadSeparationQuaternaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationPreWashSolutionVolume:

If MagneticBeadSeparationQuaternaryWash is set to True and MagneticBeadSeparationPreWash is set to False, MagneticBeadSeparationQuaternaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationSampleVolume:

MagneticBeadSeparationQuinaryWash (2)

If any magnetic bead separation quinary wash options are set (MagneticBeadSeparationQuinaryWashSolution, MagneticBeadSeparationQuinaryWashSolutionVolume, MagneticBeadSeparationQuinaryWashMix, etc.), MagneticBeadSeparationQuinaryWash is automatically set to True:

If no magnetic bead separation quinary wash options are set (MagneticBeadSeparationQuinaryWashSolution, MagneticBeadSeparationQuinaryWashSolutionVolume, MagneticBeadSeparationQuinaryWashMix, etc.), MagneticBeadSeparationQuinaryWash is automatically set to False:

MagneticBeadSeparationQuinaryWashAirDry (1)

If MagneticBeadSeparationQuinaryWash is set to True, MagneticBeadSeparationQuinaryWashSolution is automatically set to Model[Sample,"Zyppy Wash Buffer"], MagneticBeadSeparationQuinaryWashMix is automatically set to True, QuinaryWashMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationQuinaryWashCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationQuinaryWashCollectionStorageCondition is automatically set to Refrigerator, NumberOfMagneticBeadSeparationQuinaryWashes is automatically set to 1, MagneticBeadSeparationQuinaryWashAirDry is automatically set to False, and MagneticBeadSeparationQuinaryWashCollectionContainerLabel is automatically generated unless otherwise specified:

MagneticBeadSeparationQuinaryWashAirDryTime (1)

If MagneticBeadSeparationQuinaryWashAirDry is set to True, MagneticBeadSeparationQuinaryWashAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationQuinaryWashAspirationVolume (1)

If MagneticBeadSeparationQuinaryWash is set to True, MagneticBeadSeparationQuinaryWashAspirationVolume is automatically set the same as the MagneticBeadSeparationQuinaryWashSolutionVolume:

MagneticBeadSeparationQuinaryWashCollectionContainer (1)

MagneticBeadSeparationQuinaryWashCollectionContainerLabel (1)

MagneticBeadSeparationQuinaryWashCollectionStorageCondition (1)

MagneticBeadSeparationQuinaryWashMix (1)

MagneticBeadSeparationQuinaryWashMixRate (1)

If MagneticBeadSeparationQuinaryWashMixType is set to Shake, MagneticBeadSeparationQuinaryWashMixTime is automatically set to 5 minutes and MagneticBeadSeparationQuinaryWashMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationQuinaryWashMixTemperature (1)

If MagneticBeadSeparationQuinaryWashMix is set to True, MagneticBeadSeparationQuinaryWashMixVolume is automatically set to Ambient:

MagneticBeadSeparationQuinaryWashMixTime (1)

MagneticBeadSeparationQuinaryWashMixTipMaterial (1)

If MagneticBeadSeparationQuinaryWashMixType is set to Pipette, NumberOfMagneticBeadSeparationQuinaryWashMixes is automatically set to 10, MagneticBeadSeparationQuinaryWashMixTipType is automatically set to WideBore, and MagneticBeadSeparationQuinaryWashMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationQuinaryWashMixTipType (1)

MagneticBeadSeparationQuinaryWashMixType (3)

If MagneticBeadSeparationQuinaryWashMixRate or MagneticBeadSeparationQuinaryWashMixTime are set, MagneticBeadSeparationQuinaryWashMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationQuinaryWashMixes or MagneticBeadSeparationQuinaryWashMixVolume are set, MagneticBeadSeparationQuinaryWashMixType is automatically set to Pipette:

If no magnetic bead separation quinary wash mix options are set, MagneticBeadSeparationQuinaryWashMixType is automatically set to Pipette:

MagneticBeadSeparationQuinaryWashMixVolume (2)

If MagneticBeadSeparationQuinaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationQuinaryWashSolution and magnetic beads volume is less than 970 microliters, MagneticBeadSeparationQuinaryWashMixVolume is automatically set to 1/2 of the combined MagneticBeadSeparationQuinaryWashSolution and magnetic beads volume:

If MagneticBeadSeparationQuinaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationQuinaryWashSolution and magnetic beads volume is greater than 970 microliters, MagneticBeadSeparationQuinaryWashMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationQuinaryWashSolution (1)

MagneticBeadSeparationQuinaryWashSolutionVolume (2)

If MagneticBeadSeparationQuinaryWash and MagneticBeadSeparationPreWash are set to True, MagneticBeadSeparationQuinaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationPreWashSolutionVolume:

If MagneticBeadSeparationQuinaryWash is set to True and MagneticBeadSeparationPreWash is set to False, MagneticBeadSeparationQuinaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationSampleVolume:

MagneticBeadSeparationSampleVolume (2)

If the volume of the sample is less than 1 Millilter, then all of the sample will be used as the MagneticBeadSeparationSampleVolume:

If the volume of the sample is greater than 1 Milliliter, 1 Milliliter will be used as the MagneticBeadSeparationSampleVolume:

MagneticBeadSeparationSecondaryWash (2)

If any magnetic bead separation secondary wash options are set (MagneticBeadSeparationSecondaryWashSolution, MagneticBeadSeparationSecondaryWashSolutionVolume, MagneticBeadSeparationSecondaryWashMix, etc.), MagneticBeadSeparationSecondaryWash is automatically set to True:

If no magnetic bead separation secondary wash options are set (MagneticBeadSeparationSecondaryWashSolution, MagneticBeadSeparationSecondaryWashSolutionVolume, MagneticBeadSeparationSecondaryWashMix, etc.), MagneticBeadSeparationSecondaryWash is automatically set to False:

MagneticBeadSeparationSecondaryWashAirDry (1)

If MagneticBeadSeparationSecondaryWash is set to True, MagneticBeadSeparationSecondaryWashSolution is automatically set to Model[Sample,"Zyppy Wash Buffer"], MagneticBeadSeparationSecondaryWashMix is automatically set to True, SecondaryWashMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationSecondaryWashCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationSecondaryWashCollectionStorageCondition is automatically set to Refrigerator, NumberOfMagneticBeadSeparationSecondaryWashes is automatically set to 1, MagneticBeadSeparationSecondaryWashAirDry is automatically set to False, and MagneticBeadSeparationSecondaryWashCollectionContainerLabel is automatically generated unless otherwise specified:

MagneticBeadSeparationSecondaryWashAirDryTime (1)

If MagneticBeadSeparationSecondaryWashAirDry is set to True, MagneticBeadSeparationSecondaryWashAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationSecondaryWashAspirationVolume (1)

If MagneticBeadSeparationSecondaryWash is set to True, MagneticBeadSeparationSecondaryWashAspirationVolume is automatically set the same as the MagneticBeadSeparationSecondaryWashSolutionVolume:

MagneticBeadSeparationSecondaryWashCollectionContainer (1)

MagneticBeadSeparationSecondaryWashCollectionContainerLabel (1)

MagneticBeadSeparationSecondaryWashCollectionStorageCondition (1)

MagneticBeadSeparationSecondaryWashMix (1)

MagneticBeadSeparationSecondaryWashMixRate (1)

If MagneticBeadSeparationSecondaryWashMixType is set to Shake, MagneticBeadSeparationSecondaryWashMixTime is automatically set to 5 minutes and MagneticBeadSeparationSecondaryWashMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationSecondaryWashMixTemperature (1)

If MagneticBeadSeparationSecondaryWashMix is set to True, MagneticBeadSeparationSecondaryWashMixVolume is automatically set to Ambient:

MagneticBeadSeparationSecondaryWashMixTime (1)

MagneticBeadSeparationSecondaryWashMixTipMaterial (1)

If MagneticBeadSeparationSecondaryWashMixType is set to Pipette, NumberOfMagneticBeadSeparationSecondaryWashMixes is automatically set to 10, MagneticBeadSeparationSecondaryWashMixTipType is automatically set to WideBore, and MagneticBeadSeparationSecondaryWashMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationSecondaryWashMixTipType (1)

MagneticBeadSeparationSecondaryWashMixType (3)

If MagneticBeadSeparationSecondaryWashMixRate or MagneticBeadSeparationSecondaryWashMixTime are set, MagneticBeadSeparationSecondaryWashMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationSecondaryWashMixes or MagneticBeadSeparationSecondaryWashMixVolume are set, MagneticBeadSeparationSecondaryWashMixType is automatically set to Pipette:

If no magnetic bead separation secondary wash mix options are set, MagneticBeadSeparationSecondaryWashMixType is automatically set to Pipette:

MagneticBeadSeparationSecondaryWashMixVolume (2)

If MagneticBeadSeparationSecondaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationSecondaryWashSolution and magnetic beads volume is less than 970 microliters, MagneticBeadSeparationSecondaryWashMixVolume is automatically set to 1/2 of the combined MagneticBeadSeparationSecondaryWashSolution and magnetic beads volume:

If MagneticBeadSeparationSecondaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationSecondaryWashSolution and magnetic beads volume is greater than 970 microliters, MagneticBeadSeparationSecondaryWashMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationSecondaryWashSolution (1)

MagneticBeadSeparationSecondaryWashSolutionVolume (2)

If MagneticBeadSeparationSecondaryWash and MagneticBeadSeparationPreWash are set to True, MagneticBeadSeparationSecondaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationPreWashSolutionVolume:

If MagneticBeadSeparationSecondaryWash is set to True and MagneticBeadSeparationPreWash is set to False, MagneticBeadSeparationSecondaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationSampleVolume:

MagneticBeadSeparationSelectionStrategy (1)

MagneticBeadSeparationSenaryWash (2)

If any magnetic bead separation senary wash options are set (MagneticBeadSeparationSenaryWashSolution, MagneticBeadSeparationSenaryWashSolutionVolume, MagneticBeadSeparationSenaryWashMix, etc.), MagneticBeadSeparationSenaryWash is automatically set to True:

If no magnetic bead separation senary wash options are set (MagneticBeadSeparationSenaryWashSolution, MagneticBeadSeparationSenaryWashSolutionVolume, MagneticBeadSeparationSenaryWashMix, etc.), MagneticBeadSeparationSenaryWash is automatically set to False:

MagneticBeadSeparationSenaryWashAirDry (1)

If MagneticBeadSeparationSenaryWash is set to True, MagneticBeadSeparationSenaryWashSolution is automatically set to Model[Sample,"Zyppy Wash Buffer"], MagneticBeadSeparationSenaryWashMix is automatically set to True, SenaryWashMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationSenaryWashCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationSenaryWashCollectionStorageCondition is automatically set to Refrigerator, NumberOfMagneticBeadSeparationSenaryWashes is automatically set to 1, MagneticBeadSeparationSenaryWashAirDry is automatically set to False, and MagneticBeadSeparationSenaryWashCollectionContainerLabel is automatically generated unless otherwise specified:

MagneticBeadSeparationSenaryWashAirDryTime (1)

If MagneticBeadSeparationSenaryWashAirDry is set to True, MagneticBeadSeparationSenaryWashAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationSenaryWashAspirationVolume (1)

If MagneticBeadSeparationSenaryWash is set to True, MagneticBeadSeparationSenaryWashAspirationVolume is automatically set the same as the MagneticBeadSeparationSenaryWashSolutionVolume:

MagneticBeadSeparationSenaryWashCollectionContainer (1)

MagneticBeadSeparationSenaryWashCollectionContainerLabel (1)

MagneticBeadSeparationSenaryWashCollectionStorageCondition (1)

MagneticBeadSeparationSenaryWashMix (1)

MagneticBeadSeparationSenaryWashMixRate (1)

If MagneticBeadSeparationSenaryWashMixType is set to Shake, MagneticBeadSeparationSenaryWashMixTime is automatically set to 5 minutes and MagneticBeadSeparationSenaryWashMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationSenaryWashMixTemperature (1)

If MagneticBeadSeparationSenaryWashMix is set to True, MagneticBeadSeparationSenaryWashMixVolume is automatically set to Ambient:

MagneticBeadSeparationSenaryWashMixTime (1)

MagneticBeadSeparationSenaryWashMixTipMaterial (1)

If MagneticBeadSeparationSenaryWashMixType is set to Pipette, NumberOfMagneticBeadSeparationSenaryWashMixes is automatically set to 10, MagneticBeadSeparationSenaryWashMixTipType is automatically set to WideBore, and MagneticBeadSeparationSenaryWashMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationSenaryWashMixTipType (1)

MagneticBeadSeparationSenaryWashMixType (3)

If MagneticBeadSeparationSenaryWashMixRate or MagneticBeadSeparationSenaryWashMixTime are set, MagneticBeadSeparationSenaryWashMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationSenaryWashMixes or MagneticBeadSeparationSenaryWashMixVolume are set, MagneticBeadSeparationSenaryWashMixType is automatically set to Pipette:

If no magnetic bead separation senary wash mix options are set, MagneticBeadSeparationSenaryWashMixType is automatically set to Pipette:

MagneticBeadSeparationSenaryWashMixVolume (2)

If MagneticBeadSeparationSenaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationSenaryWashSolution and magnetic beads volume is less than 970 microliters, MagneticBeadSeparationSenaryWashMixVolume is automatically set to 1/2 of the combined MagneticBeadSeparationSenaryWashSolution and magnetic beads volume:

If MagneticBeadSeparationSenaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationSenaryWashSolution and magnetic beads volume is greater than 970 microliters, MagneticBeadSeparationSenaryWashMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationSenaryWashSolution (1)

MagneticBeadSeparationSenaryWashSolutionVolume (2)

If MagneticBeadSeparationSenaryWash and MagneticBeadSeparationPreWash are set to True, MagneticBeadSeparationSenaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationPreWashSolutionVolume:

If MagneticBeadSeparationSenaryWash is set to True and MagneticBeadSeparationPreWash is set to False, MagneticBeadSeparationSenaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationSampleVolume:

MagneticBeadSeparationSeptenaryWash (2)

If any magnetic bead separation septenary wash options are set (MagneticBeadSeparationSeptenaryWashSolution, MagneticBeadSeparationSeptenaryWashSolutionVolume, MagneticBeadSeparationSeptenaryWashMix, etc.), MagneticBeadSeparationSeptenaryWash is automatically set to True:

If no magnetic bead separation septenary wash options are set (MagneticBeadSeparationSeptenaryWashSolution, MagneticBeadSeparationSeptenaryWashSolutionVolume, MagneticBeadSeparationSeptenaryWashMix, etc.), MagneticBeadSeparationSeptenaryWash is automatically set to False:

MagneticBeadSeparationSeptenaryWashAirDry (1)

If MagneticBeadSeparationSeptenaryWash is set to True, MagneticBeadSeparationSeptenaryWashSolution is automatically set to Model[Sample,"Zyppy Wash Buffer"], MagneticBeadSeparationSeptenaryWashCollectionContainerLabel is automatically generated, MagneticBeadSeparationSeptenaryWashMix is automatically set to True, SeptenaryWashMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationSeptenaryWashCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationSeptenaryWashCollectionStorageCondition is automatically set to Refrigerator,MagneticBeadSeparationSeptenaryWashAirDry is automatically set to False, and NumberOfMagneticBeadSeparationSeptenaryWashes is automatically set to 1 unless otherwise specified:

MagneticBeadSeparationSeptenaryWashAirDryTime (1)

If MagneticBeadSeparationSeptenaryWashAirDry is set to True, MagneticBeadSeparationSeptenaryWashAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationSeptenaryWashAspirationVolume (1)

If MagneticBeadSeparationSeptenaryWash is set to True, MagneticBeadSeparationSeptenaryWashAspirationVolume is automatically set the same as the MagneticBeadSeparationSeptenaryWashSolutionVolume:

MagneticBeadSeparationSeptenaryWashCollectionContainer (1)

MagneticBeadSeparationSeptenaryWashCollectionContainerLabel (1)

MagneticBeadSeparationSeptenaryWashCollectionStorageCondition (1)

MagneticBeadSeparationSeptenaryWashMix (1)

MagneticBeadSeparationSeptenaryWashMixRate (1)

If MagneticBeadSeparationSeptenaryWashMixType is set to Shake, MagneticBeadSeparationSeptenaryWashMixTime is automatically set to 5 minutes and MagneticBeadSeparationSeptenaryWashMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationSeptenaryWashMixTemperature (1)

If MagneticBeadSeparationSeptenaryWashMix is set to True, MagneticBeadSeparationSeptenaryWashMixVolume is automatically set to Ambient:

MagneticBeadSeparationSeptenaryWashMixTime (1)

MagneticBeadSeparationSeptenaryWashMixTipMaterial (1)

If MagneticBeadSeparationSeptenaryWashMixType is set to Pipette, NumberOfMagneticBeadSeparationSeptenaryWashMixes is automatically set to 10, MagneticBeadSeparationSeptenaryWashMixTipType is automatically set to WideBore, and MagneticBeadSeparationSeptenaryWashMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationSeptenaryWashMixTipType (1)

MagneticBeadSeparationSeptenaryWashMixType (3)

If MagneticBeadSeparationSeptenaryWashMixRate or MagneticBeadSeparationSeptenaryWashMixTime are set, MagneticBeadSeparationSeptenaryWashMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationSeptenaryWashMixes or MagneticBeadSeparationSeptenaryWashMixVolume are set, MagneticBeadSeparationSeptenaryWashMixType is automatically set to Pipette:

If no magnetic bead separation septenary wash mix options are set, MagneticBeadSeparationSeptenaryWashMixType is automatically set to Pipette:

MagneticBeadSeparationSeptenaryWashMixVolume (2)

If MagneticBeadSeparationSeptenaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationSeptenaryWashSolution and magnetic beads volume is less than 970 microliters, MagneticBeadSeparationSeptenaryWashMixVolume is automatically set to 1/2 of the combined MagneticBeadSeparationSeptenaryWashSolution and magnetic beads volume:

If MagneticBeadSeparationSeptenaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationSeptenaryWashSolution and magnetic beads volume is greater than 970 microliters, MagneticBeadSeparationSeptenaryWashMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationSeptenaryWashSolution (1)

MagneticBeadSeparationSeptenaryWashSolutionVolume (2)

If MagneticBeadSeparationSeptenaryWash and MagneticBeadSeparationPreWash are set to True, MagneticBeadSeparationSeptenaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationPreWashSolutionVolume:

If MagneticBeadSeparationSeptenaryWash is set to True and MagneticBeadSeparationPreWash is set to False, MagneticBeadSeparationSeptenaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationSampleVolume:

MagneticBeadSeparationTertiaryWash (2)

If any magnetic bead separation tertiary wash options are set (MagneticBeadSeparationTertiaryWashSolution, MagneticBeadSeparationTertiaryWashSolutionVolume, MagneticBeadSeparationTertiaryWashMix, etc.), MagneticBeadSeparationTertiaryWash is automatically set to True:

If no magnetic bead separation tertiary wash options are set (MagneticBeadSeparationTertiaryWashSolution, MagneticBeadSeparationTertiaryWashSolutionVolume, MagneticBeadSeparationTertiaryWashMix, etc.), MagneticBeadSeparationTertiaryWash is automatically set to False:

MagneticBeadSeparationTertiaryWashAirDry (1)

If MagneticBeadSeparationTertiaryWash is set to True, MagneticBeadSeparationTertiaryWashSolution is automatically set to Model[Sample,"Zyppy Wash Buffer"], MagneticBeadSeparationTertiaryWashMix is automatically set to True, TertiaryWashMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationTertiaryWashCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationTertiaryWashCollectionStorageCondition is automatically set to Refrigerator, NumberOfMagneticBeadSeparationTertiaryWashes is automatically set to 1, MagneticBeadSeparationTertiaryWashAirDry is automatically set to False, and MagneticBeadSeparationTertiaryWashCollectionContainerLabel is automatically generated unless otherwise specified:

MagneticBeadSeparationTertiaryWashAirDryTime (1)

If MagneticBeadSeparationTertiaryWashAirDry is set to True, MagneticBeadSeparationTertiaryWashAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationTertiaryWashAspirationVolume (1)

If MagneticBeadSeparationTertiaryWash is set to True, MagneticBeadSeparationTertiaryWashAspirationVolume is automatically set the same as the MagneticBeadSeparationTertiaryWashSolutionVolume:

MagneticBeadSeparationTertiaryWashCollectionContainer (1)

MagneticBeadSeparationTertiaryWashCollectionContainerLabel (1)

MagneticBeadSeparationTertiaryWashCollectionStorageCondition (1)

MagneticBeadSeparationTertiaryWashMix (1)

MagneticBeadSeparationTertiaryWashMixRate (1)

If MagneticBeadSeparationTertiaryWashMixType is set to Shake, MagneticBeadSeparationTertiaryWashMixTime is automatically set to 5 minutes and MagneticBeadSeparationTertiaryWashMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationTertiaryWashMixTemperature (1)

If MagneticBeadSeparationTertiaryWashMix is set to True, MagneticBeadSeparationTertiaryWashMixVolume is automatically set to Ambient:

MagneticBeadSeparationTertiaryWashMixTime (1)

MagneticBeadSeparationTertiaryWashMixTipMaterial (1)

If MagneticBeadSeparationTertiaryWashMixType is set to Pipette, NumberOfMagneticBeadSeparationTertiaryWashMixes is automatically set to 10, MagneticBeadSeparationTertiaryWashMixTipType is automatically set to WideBore, and MagneticBeadSeparationTertiaryWashMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationTertiaryWashMixTipType (1)

MagneticBeadSeparationTertiaryWashMixType (3)

If MagneticBeadSeparationTertiaryWashMixRate or MagneticBeadSeparationTertiaryWashMixTime are set, MagneticBeadSeparationTertiaryWashMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationTertiaryWashMixes or MagneticBeadSeparationTertiaryWashMixVolume are set, MagneticBeadSeparationTertiaryWashMixType is automatically set to Pipette:

If no magnetic bead separation tertiary wash mix options are set, MagneticBeadSeparationTertiaryWashMixType is automatically set to Pipette:

MagneticBeadSeparationTertiaryWashMixVolume (2)

If MagneticBeadSeparationTertiaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationTertiaryWashSolution and magnetic beads volume is less than 970 microliters, MagneticBeadSeparationTertiaryWashMixVolume is automatically set to 1/2 of the combined MagneticBeadSeparationTertiaryWashSolution and magnetic beads volume:

If MagneticBeadSeparationTertiaryWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationTertiaryWashSolution and magnetic beads volume is greater than 970 microliters, MagneticBeadSeparationTertiaryWashMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationTertiaryWashSolution (1)

MagneticBeadSeparationTertiaryWashSolutionVolume (2)

If MagneticBeadSeparationTertiaryWash and MagneticBeadSeparationPreWash are set to True, MagneticBeadSeparationTertiaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationPreWashSolutionVolume:

If MagneticBeadSeparationTertiaryWash is set to True and MagneticBeadSeparationPreWash is set to False, MagneticBeadSeparationTertiaryWashSolutionVolume is automatically set to the same value as the MagneticBeadSeparationSampleVolume:

MagneticBeadSeparationWash (2)

If any magnetic bead separation wash options are set (MagneticBeadSeparationWashSolution, MagneticBeadSeparationWashSolutionVolume, MagneticBeadSeparationWashMix, etc.), MagneticBeadSeparationWash is automatically set to True:

If no magnetic bead separation wash options are set (MagneticBeadSeparationWashSolution, MagneticBeadSeparationWashSolutionVolume, MagneticBeadSeparationWashMix, etc.), MagneticBeadSeparationWash is automatically set to False:

MagneticBeadSeparationWashAirDry (1)

If MagneticBeadSeparationWash is set to True, MagneticBeadSeparationWashSolution is automatically set to Model[Sample,"Zyppy Wash Buffer"], MagneticBeadSeparationWashMix is automatically set to True, WashMagnetizationTime is automatically set to 5 minutes, MagneticBeadSeparationWashCollectionContainer is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"], MagneticBeadSeparationWashCollectionStorageCondition is automatically set to Refrigerator, NumberOfMagneticBeadSeparationWashes is automatically set to 1, MagneticBeadSeparationWashAirDry is automatically set to False, and MagneticBeadSeparationWashCollectionContainerLabel is automatically generated unless otherwise specified:

MagneticBeadSeparationWashAirDryTime (1)

If MagneticBeadSeparationWashAirDry is set to True, MagneticBeadSeparationWashAirDryTime is automatically set to 1 Minute:

MagneticBeadSeparationWashAspirationVolume (1)

If MagneticBeadSeparationWash is set to True, MagneticBeadSeparationWashAspirationVolume is automatically set the same as the MagneticBeadSeparationWashSolutionVolume:

MagneticBeadSeparationWashCollectionContainer (1)

MagneticBeadSeparationWashCollectionContainerLabel (1)

MagneticBeadSeparationWashCollectionStorageCondition (1)

MagneticBeadSeparationWashMix (1)

MagneticBeadSeparationWashMixRate (1)

If MagneticBeadSeparationWashMixType is set to Shake, MagneticBeadSeparationWashMixTime is automatically set to 5 minutes and MagneticBeadSeparationWashMixRate is automatically set to 300 RPM unless otherwise specified:

MagneticBeadSeparationWashMixTemperature (1)

If MagneticBeadSeparationWashMix is set to True, MagneticBeadSeparationWashMixVolume is automatically set to Ambient:

MagneticBeadSeparationWashMixTime (1)

MagneticBeadSeparationWashMixTipMaterial (1)

If MagneticBeadSeparationWashMixType is set to Pipette, NumberOfMagneticBeadSeparationWashMixes is automatically set to 10, MagneticBeadSeparationWashMixTipType is automatically set to WideBore and MagneticBeadSeparationWashMixTipMaterial is automatically set to Polypropylene unless otherwise specified:

MagneticBeadSeparationWashMixTipType (1)

MagneticBeadSeparationWashMixType (3)

If MagneticBeadSeparationWashMixRate or MagneticBeadSeparationWashMixTime are set, MagneticBeadSeparationWashMixType is automatically set to Shake:

If NumberOfMagneticBeadSeparationWashMixes or MagneticBeadSeparationWashMixVolume are set, MagneticBeadSeparationWashMixType is automatically set to Pipette:

If no magnetic bead separation equilibration mix options are set, MagneticBeadSeparationWashMixType is automatically set to Pipette:

MagneticBeadSeparationWashMixVolume (2)

If MagneticBeadSeparationWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationWashSolution and magnetic beads volume is less than 970 microliters, MagneticBeadSeparationWashMixVolume is automatically set to 1/2 of the combined MagneticBeadSeparationWashSolution and magnetic beads volume:

If MagneticBeadSeparationWashMixType is set to Pipette and 1/2 of the combined MagneticBeadSeparationWashSolution and magnetic beads volume is greater than 970 microliters, MagneticBeadSeparationWashMixVolume is automatically set to 970 microliters:

MagneticBeadSeparationWashSolution (1)

MagneticBeadSeparationWashSolutionVolume (1)

If MagneticBeadSeparationWash is set to True, MagneticBeadSeparationWashSolution is automatically set to the same value as the MagneticBeadSeparationPreWashSolutionVolume:

MagneticBeadVolume (1)

MagneticBeadVolume is automatically set to 1/10 of the MagneticBeadSeparationSampleVolume if not otherwise specified:

MagnetizationRack (1)

Method (1)

If a plasmid DNA extraction method is specified, then the options from the method object will be used to determine other options:

NeutralizationFilter (2)

If NeutralizationSeparationTechnique is set to Filter and the sample is already in a filter, then NeutralizationFilter is set to the filter that the sample is already in and NeutralizationFilterPosition is set to the position that the sample is in the filter unless otherwise specified:

If NeutralizationSeparationTechnique is set to Filter and the sample is not already in a filter, NeutralizationFilter is automatically set to a filter that fits on the filtration instrument, either the centrifuge or pressure manifold and matches MembraneMaterial and PoreSize if they are set and NeutralizationFilterPosition is set to the first available position in the NeutralizationFilter unless otherwise specified:

NeutralizationFilterCentrifugeIntensity (1)

If NeutralizationFiltrationTechnique is set to centrifuge, then NeutralizationFiltrationInstrument is automatically set to Model[Instrument,Centrifuge,"HiG4"] and NeutralizationFilterCentrifugeIntensity is automatically set to 3600 G unless otherwise specified:

NeutralizationFilterPosition (2)

NeutralizationFilterStorageCondition (1)

If NeutralizationSeparationTechnique is set to Filter, then NeutralizationFiltrationTechnique is automatically set to AirPressure, NeutralizationFiltrationTime is automatically set to 10 minutes, and NeutralizationFilterStorageCondition is set to Disposal unless otherwise specified:

NeutralizationFiltrationInstrument (2)

If NeutralizationFiltrationTechnique is set to AirPressure, then NeutralizationFiltrationInstrument is automatically set to Model[Instrument, PressureManifold, "MPE2 Sterile"] and NeutralizationFiltrationPressure is automatically set to 40 PSI unless otherwise specified:

NeutralizationFiltrationPressure (1)

NeutralizationFiltrationTechnique (1)

NeutralizationFiltrationTime (1)

NeutralizationMembraneMaterial (2)

If a neutralization filter is specified, then NeutralizationPoreSize is set to the pore size of the specified filter and NeutralizationMembraneMaterial is set to the membrane material of the specified filter:

If the neutralization separation technique is set to filter and a filter is not specified, then the neutralization membrane material will be set to the material of the resolved filter:

NeutralizationMixInstrument (2)

If the neutralization mixture will be mixed by shaking, the mixing temperature is less than or equal to 70 degrees Celsius, and the mixing rate is outside the range of 400-1800 RPM, then the mixing instrument is set to Model[Instrument, Shaker, "Inheco ThermoshakeAC"]:

If the neutralization mixture will be mixed by shaking and the mixing temperature is greater than 70 degrees Celsius and the mixing rate is within 400-1800 RPM, then the mixing instrument is set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"]:

NeutralizationMixRate (1)

If NeutralizationMixType is set to Shake, then the NeutralizationMixRate is automatically set to 300 RPM (revolutions per minute) and NeutralizationMixTime is automatically set to 15 minutes unless otherwise specified:

NeutralizationMixTemperature (1)

If the neutralization mixture will be mixed, then the mixing temperature is set to ambient temperature by default:

NeutralizationMixTime (1)

NeutralizationMixType (3)

If a mixing time or mixing rate for the neutralization is specified, then the sample will be shaken (since a mixing time or rate does not pertain to pipette mixing):

If a mixing volume or number of mixes for the neutralization is specified, then the sample will be mixed with a pipette (since a mixing volume and number of mixes do not pertain to shaking):

If mixing options are not specified for neutralization, then the sample will not be mixed by default:

NeutralizationMixVolume (2)

If half of the neutralization mixture (the sample volume plus the added neutralization reagent volume) is less than 970 microliters, then that volume will be used as the mixing volume for the pipette mixing:

If half of the neutralization mixture (the sample volume plus the added neutralization reagent volume) is greater than 970 microliters, then 970 microliters (the maximum amount that can be mixed via pipette on the liquid handling robot) will be used as the mixing volume for the pipette mixing:

NeutralizationPelletCentrifugeIntensity (1)

If NeutralizationSeparationTechnique is set to Pellet, NeutralizationPelletVolume is automatically set to 1 microliter, NeutralizationPelletInstrument is automatically set to Model[Instrument, Centrifuge, "HiG4"], NeutralizationPelletCentrifugeIntensity is automatically set to 3600 G, NeutralizationPelletCentrifugeTime is automatically set to 10 minutes, and NeutralizedPelletStorageCondition is set to Disposal unless otherwise specified:

NeutralizationPelletCentrifugeTime (1)

NeutralizationPelletInstrument (1)

NeutralizationPelletVolume (1)

NeutralizationPoreSize (1)

NeutralizationReagent (1)

By default, if a sample will be neutralized, then NeutralizationReagent will automatically be set to Model[Sample, StockSolution, "3M Sodium Acetate"]], NeutralizationReagentTemperature will be automatically set to Ambient, NeutralizationReagentEquilibrationTime will automatically be set to 5 minutes, and NeutralizationSettlingTime will be set to 15 minutes unless otherwise specified:

NeutralizationReagentEquilibrationTime (1)

NeutralizationReagentTemperature (1)

NeutralizationReagentVolume (2)

If half of the lysate volume is less than 80% of the maximum volume of the container minus the volume of the lysate, then half of the lysate volume is used as the volume of the neutralization reagent added to the lysate:

If half of the lysate volume is greater than 80% of the maximum volume of the container minus the volume of the lysate, then 80% of the maximum volume of the container minus the volume of the lysate is used as the volume of the neutralization reagent added to the lysate:

NeutralizationSeparationTechnique (2)

If any neutralization filtration options are specified, then the sample will be filtered to separate the neutralized supernatant from the cell debris:

If no neutralization filtration options are specified, then the sample will be pelleted to separate the neutralized supernatant from the cell debris by default:

NeutralizationSettlingInstrument (1)

If the neutralization settling time is greater than 0 minutes, then NeutralizationSettlingInstrument is automatically set to Model[Instrument, HeatBlock, "Hamilton Heater Cooler"] and NeutralizationSettlingTemperature is automatically set to Ambient unless otherwise specified:

NeutralizationSettlingTemperature (1)

NeutralizationSettlingTime (1)

Neutralize (4)

If a sample is being lysed, it will also be neutralized (since lysate often needs to be neutralized soon after lysis for plasmid DNA extraction):

If any neutralization options are set, then the sample will be neutralized:

If the sample is a lysate, then the sample will be neutralized:

If the sample is not a lysate, not being lysed, nor has any neutralization options specified, then the sample will not be neutralized:

NeutralizedPelletContainerLabel (1)

If NeutralizedPelletStorageCondition is not set to Disposal, NeutralizedPelletLabel and NeutralizedPelletContainerLabel are automatically generated for the neutralized pellet sample unless otherwise specified:

NeutralizedPelletLabel (1)

NeutralizedPelletStorageCondition (1)

NeutralizedSupernatantTransferVolume (1)

If the separation technique for the sample will be pelleting, then the amount of supernatant transferred from the centrifuged neutralization solution will be 90% of the neutralization reagent plus the sample volume by default:

NumberOfLiquidLiquidExtractionMixes (1)

If tLiquidLiquidExtractionMixType is set to Pipette, then NumberOfLiquidLiquidExtractionMixes is automatically set to 10:

NumberOfLiquidLiquidExtractions (1)

NumberOfLysisMixes (1)

If LysisMixType is set to Pipette, NumberOfLysisMixes is automatically set to 10:

NumberOfLysisSteps (3)

If any tertiary lysis steps are set, then NumberOfLysisSteps will be set to 3:

If any secondary lysis steps are specified but no tertiary lysis steps are set, then NumberOfLysisSteps will be set to 2:

If no secondary nor tertiary lysis steps are set, then NumberOfLysisSteps will be set to 1:

NumberOfMagneticBeadSeparationElutionMixes (1)

NumberOfMagneticBeadSeparationElutions (1)

NumberOfMagneticBeadSeparationEquilibrationMixes (1)

NumberOfMagneticBeadSeparationLoadingMixes (1)

NumberOfMagneticBeadSeparationPreWashes (1)

NumberOfMagneticBeadSeparationPreWashMixes (1)

NumberOfMagneticBeadSeparationQuaternaryWashes (1)

NumberOfMagneticBeadSeparationQuaternaryWashMixes (1)

NumberOfMagneticBeadSeparationQuinaryWashes (1)

NumberOfMagneticBeadSeparationQuinaryWashMixes (1)

NumberOfMagneticBeadSeparationSecondaryWashes (1)

NumberOfMagneticBeadSeparationSecondaryWashMixes (1)

NumberOfMagneticBeadSeparationSenaryWashes (1)

NumberOfMagneticBeadSeparationSenaryWashMixes (1)

NumberOfMagneticBeadSeparationSeptenaryWashes (1)

NumberOfMagneticBeadSeparationSeptenaryWashMixes (1)

NumberOfMagneticBeadSeparationTertiaryWashes (1)

NumberOfMagneticBeadSeparationTertiaryWashMixes (1)

NumberOfMagneticBeadSeparationWashes (1)

NumberOfMagneticBeadSeparationWashMixes (1)

NumberOfNeutralizationMixes (1)

If the neutralization mixture will be mixed by pipette, then the number of mixes (number of times the neutralization mixture is pipetted up and down) is set to 10 by default:

NumberOfPrecipitationMixes (1)

If PrecipitationMixType is set to Pipette, then NumberOfPrecipitationMixes is automatically set to 10 :

OrganicSolvent (4)

If LiquidLiquidExtractionTargetPhase is set to Organic and the selection strategy is set to Positive (implying addition of organic solvent during later extraction rounds), then OrganicSolvent will be set to Model[Sample, "Phenol: Chloroform: Iso-Amyl Alcohol (25:24:1) Mixture"] (a phenol:chloroform:iso-amyl alcohol mixture often used in phenol-chloroform extractions):

If the target phase is set to Aqueous and the selection strategy is set to Positive (implying addition of organic solvent during later extraction rounds), then OrganicSolvent will be set to Model[Sample, "Phenol: Chloroform: Iso-Amyl Alcohol (25:24:1) Mixture"] (a phenol:chloroform:iso-amyl alcohol mixture often used in phenol-chloroform extractions):

If organic solvent is otherwise required for an extraction and LiquidLiquidExtractionTechnique is set to Pipette, then ethyl acetate (Model[Sample, "Ethyl acetate, HPLC Grade"]) will be used as the organic solvent:

If organic solvent is otherwise required for an extraction and LiquidLiquidExtractionTechnique is set to PhaseSeparator, Model[Sample, "Chloroform"] will be used if Model[Sample, "Ethyl acetate, HPLC Grade"] is not dense enough to be denser than the aqueous layer, since the organic layer needs to be below the aqueous layer to pass through the hydrophobic frit:

OrganicSolventRatio (2)

If an OrganicSolventVolume is set, then the OrganicSolventRatio is calculated from the set OrganicSolventVolume and the sample volume (SampleVolume/OrganicSolventVolume):

If an organic solvent is set, then the organic solvent ratio is set to 5:

OrganicSolventVolume (2)

If an OrganicSolventRatio is set, then the OrganicSolventRatio is calculated from the ratio of the sample volume (SampleVolume/OrganicSolventRatio):

If an OrganicSolvent is set and an OrganicSolventVolume is not set, then the OrganicSolventVolume will be automatically set to 20% of the sample volume:

PostClarificationPelletLabel (1)

PostClarificationPelletStorageCondition (1)

PrecipitatedSampleContainerLabel (1)

If PrecipitationTargetPhase is set to Solid, PrecipitationReagent is automatically set to Model[Sample,"Isopropanol"], PrecipitationNumberOfWashes is automatically set to 3, PrecipitationDryingTemperature is automatically set to Ambient, PrecipitationDryingTime is automatically set to 20 Minutes, PrecipitationResuspensionBuffer is automatically set to Model[Sample, StockSolution, "1x TE Buffer"], PrecipitatedSampleStorageCondition is set to the StorageCondition of the Sample, PrecipitatedSampleLabel is automatically generated, and PrecipitatedSampleContainerLabel is automatically generated unless otherwise specified:

PrecipitatedSampleContainerOut (1)

If a PrecipitationResuspensionBuffer is specified, PrecipitationTargetPhase is set to Solid, and PrecipitationSeparationTechnique is set to Filter, then PrecipitatedSampleContainerOut is automatically set by the PreferredContainer function based on the sample volume and the PrecipitationResuspensionBufferVolume:

PrecipitatedSampleLabel (1)

PrecipitatedSampleStorageCondition (1)

PrecipitationDryingTemperature (1)

PrecipitationDryingTime (1)

PrecipitationFilter (2)

If SeparationTechnique is set to Filter and the sample is already in a filter, PrecipitationFilter will automatically be set to the filter that the sample is in and PrecipitationFilterPosition is set to the current position of the sample in the filter unless otherwise specified:

If SeparationTechnique is set to Filter and the sample is not already in a filter, PrecipitationFilter will automatically be determined based on the SeparationTechnique and PrecipitationFiltrationInstrument and PrecipitationFilterPosition will be set to the first empty position in the PrecipitationFilter unless otherwise specified:

PrecipitationFilterCentrifugeIntensity (1)

If PrecipitationFiltrationTechnique is set to Centrifuge, PrecipitationFilterCentrifugeIntensity is automatically set to 3600 G:

PrecipitationFilterPosition (2)

PrecipitationFilterStorageCondition (2)

If PrecipitationTargetPhase is set to Solid, PrecipitationSeparationTechnique is set to Filter, and ResuspensionBuffer is not set to None, then PrecipitationFilterStorageCondition is automatically set to the StorageCondition of the sample:

If PrecipitationSeparationTechnique is set to Filter and PrecipitationTargetPhase is set to Liquid, then FilterStorageCondition is automatically set to Disposal:

PrecipitationFiltrateVolume (1)

If PrecipitationSeparationTechnique is set to Filter, PrecipitationFiltrateVolume is set to the sample volume plus PrecipitationReagentVolume:

PrecipitationFiltrationInstrument (2)

If PrecipitationSeparationTechnique is set to Filter and PrecipitationFiltrationTechnique is set to Centrifuge, PrecipitationFiltrationInstrument is automatically set to Model[Instrument, Centrifuge, "HiG4"]:

If PrecipitationSeparationTechnique is set to Filter and PrecipitationFiltrationTechnique is set to AirPressure, PrecipitationFiltrationInstrument is automatically set to Model[Instrument, Centrifuge, "MPE2 Sterile"]:

PrecipitationFiltrationPressure (1)

If PrecipitationFiltrationTechnique is set to AirPressure, PrecipitationFiltrationPressure is automatically set to 40 PSI:

PrecipitationFiltrationTechnique (1)

If PrecipitationSeparationTechnique is set to Filter, then PrecipitationFiltrationTechnique is automatically set to AirPressure and PrecipitationFiltrationTime is set to 10 Minutes:

PrecipitationFiltrationTime (1)

If PrecipitationSeparationTechnique is set to Filter, then PrecipitationFiltrationTechnique is automatically set to AirPressure and PrecipitationFiltrationTime is set to 10 Minutes:

PrecipitationInstrument (2)

If PrecipitationTemperature is set to greater than 70 Celsius, PrecipitationInstrument is automatically set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"]:

If PrecipitationTemperature is less than 70 Celsius, PrecipitationInstrument is automatically set to Model[Instrument, HeatBlock, "Hamilton Heater Cooler"]:

PrecipitationMembraneMaterial (2)

If PrecipitationSeparationTechnique is set to Filter and a PrecipitationFilter is specified,PrecipitationPoreSize is automatically set to the pore size of the specified filter and PrecipitationMembraneMaterial is automatically set to the membrane material of the specified filter:

If PrecipitationSeparationTechnique is set to Filter, a PrecipitationFilter is not specified, and a PrecipitationPoreSize is set, then PrecipitationMembraneMaterial is automatically set to PES:

PrecipitationMixInstrument (1)

If PrecipitationMixType is set to Shake, PrecipitationMixInstrument is automatically set to Model[Instrument, Shaker, "Inheco ThermoshakeAC"], PrecipitationMixRate is automatically set to 300 RPM, PrecipitationMixTemperature is automatically set to Ambient, and PrecipitationMixTime is automatically set to 15 minutes unless otherwise specified:

PrecipitationMixRate (1)

PrecipitationMixTemperature (1)

PrecipitationMixTime (1)

PrecipitationMixType (2)

If PrecipitationMixVolume or NumberOfPrecipitationMixes are specified, then PrecipitationMixType is automatically set to Pipette:

If neither PrecipitationMixVolume nor NumberOfPrecipitationMixes are specified, then PrecipitationMixType is automatically set to Shake:

PrecipitationMixVolume (2)

If PrecipitationMixType is set to Pipette and 50% of the total volume (sample volume plus the PrecipitationReagentVolume) is less than the maximum robotic transfer volume, then PrecipitationMixVolume is set to 50% of the total volume (sample volume plus the PrecipitationReagentVolume):

If PrecipitationMixType is set to Pipette and 50% of the total volume (sample volume plus the PrecipitationReagentVolume) is greater than the maximum robotic transfer volume, then PrecipitationMixVolume is set to the maximum robotic transfer volume:

PrecipitationNumberOfResuspensionMixes (1)

If PrecipitationResuspensionMixType is set to Pipette, PrecipitationNumberOfResuspensionMixes is automatically set to 10:

PrecipitationNumberOfWashes (2)

If PrecipitationTargetPhase is set to Liquid, PrecipitationReagent is automatically set to Model[Sample, StockSolution, "5M Sodium Chloride"], PrecipitationNumberOfWashes is automatically set to 0, UnprecipitatedSampleStorageCondition is set to the StorageCondition of the Sample, UnprecipitatedSampleLabel is automatically generated, and UnprecipitatedSampleContainerLabel is automatically generated unless otherwise specified:

PrecipitationNumberOfWashMixes (1)

If PrecipitationWashMixType is set to Pipette, PrecipitationNumberOfWashMixes is set to 10:

PrecipitationPelletCentrifuge (1)

If PrecipitationSeparationTechnique is set to Pellet, PrecipitationPelletVolume is automatically set to 1 MicroLiter, PrecipitationPelletCentrifuge is automatically set to Model[Instrument, Centrifuge, "HiG4"], PrecipitationPelletCentrifugeIntensity is automatically set to 3600 Gravitational Acceleration, and PrecipitationPelletCentrifugeTime is automatically set to 10 minutes unless otherwise specified:

PrecipitationPelletCentrifugeIntensity (1)

PrecipitationPelletCentrifugeTime (1)

PrecipitationPelletVolume (1)

PrecipitationPoreSize (2)

If PrecipitationSeparationTechnique is set to Filter, a PrecipitationFilter is not specified and a PrecipitationMembraneMaterial is specified, then PrecipitationPoreSize is automatically set 0.22 micron:

PrecipitationReagent (2)

PrecipitationReagentEquilibrationTime (1)

If Precipitation is included in Purification, PrecipitationTargetPhase is automatically set to Solid, PrecipitationSeparationTechnique is set to Pellet, PrecipitationReagentTemperature is automatically set to Ambient, PrecipitationReagentEquilibrationTime is automatically set to 5 minutes, and PrecipitationTime is set to 15 Minutes unless otherwise specified:

PrecipitationReagentTemperature (1)

PrecipitationReagentVolume (2)

If 50% of the sample volume is less than 80% of the sample container MaxVolume, then PrecipitationReagentVolume is set 50% of the sample volume:

If 50% of the sample volume is greater than 80% of the sample container MaxVolume minus the sample volume, then PrecipitationReagentVolume is set 80% of the sample container MaxVolume minus the sample volume:

PrecipitationResuspensionBuffer (1)

PrecipitationResuspensionBufferEquilibrationTime (1)

If a PrecipitationResuspensionBuffer is specified, PrecipitationResuspensionBufferTemperature is automatically set to Ambient and PrecipitationResuspensionBufferEquilibrationTime is automatically set to 10 minutes:

PrecipitationResuspensionBufferTemperature (1)

PrecipitationResuspensionBufferVolume (2)

If the 25% of the initial sample volume is greater than 10 microliters, then then PrecipitationResuspensionBufferVolume will be set to 25% of the initial sample volume:

If the 25% of the initial sample volume is less than 10 microliters, then PrecipitationResuspensionBufferVolume will be set to 10 microliters:

PrecipitationResuspensionMixInstrument (2)

If PrecipitationResuspensionMixType is set to Shake and PrecipitationResuspensionMixTemperature is below 70 Celsius, PrecipitationResuspensionMixInstrument is automatically set to Model[Instrument, Shaker, "Inheco ThermoshakeAC"]:

If PrecipitationResuspensionMixType is set to Shake and PrecipitationResuspensionMixTemperature is above 70 Celsius, PrecipitationResuspensionMixInstrument is automatically set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"]:

PrecipitationResuspensionMixRate (1)

If PrecipitationResuspensionMixType is set to Shake, PrecipitationResuspensionMixRate is automatically set to 300 RPM, PrecipitationResuspensionMixTemperature is automatically set to Ambient, and PrecipitationResuspensionMixTime is automatically set to 15 minutes unless otherwise specified:

PrecipitationResuspensionMixTemperature (1)

PrecipitationResuspensionMixTime (1)

PrecipitationResuspensionMixType (3)

If any resuspension pipetting options are set (PrecipitationResuspensionMixVolume and PrecipitationNumberOfResuspensionMixes), then PrecipitationResuspensionMixType is automatically set to Pipette:

If a PrecipitationResuspensionBuffer is set and PrecipitationSeparationTechnique is set to Filter, PrecipitationResuspensionMixType is set to Pipette:

If a PrecipitationResuspensionBuffer is specified, neither PrecipitationResuspensionMixVolume or PrecipitationNumberOfResuspensionMixes are set, and PrecipitationSeparationTechnique is not set to Filter, PrecipitationResuspensionMixType is automatically set to Shake:

PrecipitationResuspensionMixVolume (2)

If 50% of the PrecipitationResuspensionBufferVolume is less than the maximum robotic transfer volume, then PrecipitationResuspensionMixVolume is set to 50% of the PrecipitationResuspensionBufferVolume:

If 50% of the PrecipitationResuspensionBufferVolume is greater than the maximum robotic transfer volume, then PrecipitationResuspensionMixVolume is set to the maximum robotic transfer volume:

PrecipitationSeparationTechnique (1)

PrecipitationSupernatantVolume (1)

If PrecipitationSeparationTechnique is set to Pellet, PrecipitationSupernatantVolume is automatically set to 90% of the sample volume plus PrecipitationReagentVolume:

PrecipitationTargetPhase (1)

PrecipitationTemperature (1)

If PrecipitationTime is set to greater than 0 Minutes, then PrecipitationTemperature automatically is set to Ambient:

PrecipitationWashCentrifugeIntensity (1)

If PrecipitationSeparationTechnique is set to Pellet, PrecipitationWashCentrifugeIntensity is set to 3600 GravitationalAcceleration:

PrecipitationWashMixInstrument (2)

If PrecipitationWashMixType is set to Shake and PrecipitationWashMixTemperature is below 70 Celsius, PrecipitationWashMixInstrument is automatically set to Model[Instrument, Shaker, "Inheco ThermoshakeAC"]:

If PrecipitationWashMixType is set to Shake and PrecipitationWashMixTemperature is above 70 Celsius, PrecipitationWashMixInstrument is set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"]:

PrecipitationWashMixRate (1)

If PrecipitationWashMixType is set to Shake, PrecipitationWashMixRate is set to 300 RPM, PrecipitationWashMixTemperature is set to Ambient, and PrecipitationWashMixTime is set to 15 Minutes unless otherwise specified:

PrecipitationWashMixTemperature (1)

PrecipitationWashMixTime (1)

PrecipitationWashMixType (2)

If PrecipitationTargetPhase is set to Solid and PrecipitationSeparationTechnique is set to Filter or any pipetting options are set (PrecipitationWashMixVolume and PrecipitationNumberOfWashMixes), then PrecipitationWashMixType is automatically set to Pipette if PrecipitationNumberOfWashes is greater than 0:

If PrecipitationTargetPhase is set to Solid and pipetting options are not set (PrecipitationWashMixVolume and PrecipitationNumberOfWashMixes), then PrecipitationWashMixType is automatically set to Shake if PrecipitationNumberOfWashes is greater than 0:

PrecipitationWashMixVolume (2)

If PrecipitationWashMixType is set to Pipette and 50% of PrecipitationWashSolutionVolume is less than the maximum robotic transfer volume, then PrecipitationWashMixVolume is set to 50% of PrecipitationWashSolutionVolume:

If PrecipitationWashMixType is set to Pipette and 50% of PrecipitationWashSolutionVolume is greater than the maximum robotic transfer volume, then PrecipitationWashMixVolume is set to the maximum robotic transfer volume:

PrecipitationWashPrecipitationInstrument (2)

If PrecipitationWashPrecipitationTemperature is set to greater than 70 Celsius and PrecipitationWashPrecipitationTime is greater than 0 Minute, PrecipitationWashPrecipitationInstrument is automatically set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"]:

If PrecipitationWashPrecipitationTemperature is less than 70 Celsius and PrecipitationWashPrecipitationTime is greater than 0 Minute, PrecipitationWashPrecipitationInstrument is automatically set to Model[Instrument, HeatBlock, "Hamilton Heater Cooler"]:

PrecipitationWashPrecipitationTemperature (1)

If PrecipitationWashPrecipitationTime is greater than 0, PrecipitationWashPrecipitationTemperature is set to Ambient:

PrecipitationWashPrecipitationTime (1)

If the PrecipitationWashSolutionTemperature is greater than the PrecipitationReagentTemperature, PrecipitationWashPrecipitationTime is automatically set to 1 Minute:

PrecipitationWashPressure (1)

If PrecipitationFiltrationTechnique is set to AirPressure and PrecipitationNumberOfWashes is greater than 0, PrecipitationWashPressure is automatically set to 40 PSI and PrecipitationWashSeparationTime is set to 3 Minutes unless otherwise specified:

PrecipitationWashSeparationTime (2)

If PrecipitationFiltrationTechnique is set to Centrifuge and PrecipitationNumberOfWashes is greater than 0, PrecipitationWashSeparationTime is automatically set to 20 Minutes:

PrecipitationWashSolution (1)

If PrecipitationNumberOfWashes is greater than 0, PrecipitationWashSolution is set to Model[Sample, StockSolution, "70% Ethanol"], PrecipitationWashSolutionVolume is set to the volume of the sample, PrecipitationWashSolutionTemperature is set to Ambient, and PrecipitationWashSolutionEquilibrationTime is set to 10 minutes unless otherwise specified:

PrecipitationWashSolutionEquilibrationTime (1)

PrecipitationWashSolutionTemperature (1)

PrecipitationWashSolutionVolume (1)

PreLysisDiluent (1)

If PreLysisDilute is set to True, PreLysisDiluent is automatically set to Model[Sample, StockSolution, "1x PBS from 10X stock"]:

PreLysisDilute (1)

If either PreLysisDiluent or PreLysisDilutionVolume are set, then PreLysisDilute is automatically set to True:

PreLysisDilutionVolume (1)

If PreLysisDilute is True and a TargetCellConcentration is specified, then PreLysisDilutionVolume is set to the volume required to attain the TargetCellConcentration:

PreLysisPellet (2)

If any pre-lysis pelleting options are specified (PreLysisPelletingIntensity, PreLysisPelletingTime, PreLysisSupernatantVolume, PreLysisSupernatantStorageCondition, or PreLysisSupernatantContainer), PreLysisPellet is set to True:

If no pre-lysis pelleting options are specified (PreLysisPelletingIntensity, PreLysisPelletingTime, PreLysisSupernatantVolume, PreLysisSupernatantStorageCondition, or PreLysisSupernatantContainer), PreLysisPellet is set to False:

PreLysisPelletingCentrifuge (1)

If PreLysisPellet is set to True, PreLysisPelletCentrifuge is automatically set to Model[Instrument, Centrifuge, "HiG4"], PreLysisPelletingTime is automatically set to 10 minutes, PreLysisSupernatantVolume is automatically set to 80% of the of total sample volume, PreLysisSupernatantStorageCondition is automatically set to Disposal, PreLysisSupernatantContainer is automatically set by PackContainers, PreLysisSupernatantLabel will be automatically generated, and PreLysisSupernatantContainerLabel will be automatically generated unless otherwise specified:

PreLysisPelletingIntensity (3)

If PreLysisPellet is set to True and CellType is Mammalian, PreLysisPelletCentrifugeIntensity is automatically set to 1560 RPM:

If PreLysisPellet is set to True and CellType is Bacterial, PreLysisPelletCentrifugeIntensity is automatically set to 4030 RPM:

If PreLysisPellet is set to True and CellType is Yeast, PreLysisPelletCentrifugeIntensity is automatically set to 2850 RPM:

PreLysisPelletingTime (1)

PreLysisSupernatantContainer (1)

PreLysisSupernatantContainerLabel (1)

PreLysisSupernatantLabel (1)

PreLysisSupernatantStorageCondition (1)

PreLysisSupernatantVolume (1)

PreWashMagnetizationTime (1)

Purification (6)

If any liquid-liquid extraction options are set, then a liquid-liquid extraction will be added to the list of purification steps:

If any precipitation options are set, then precipitation will be added to the list of purification steps:

If any solid phase extraction options are set, then a solid phase extraction will be added to the list of purification steps:

If any magnetic bead separation options are set, then a magnetic bead separation will be added to the list of purification steps:

If options from multiple purification steps are specified, then they will be added to the purification step list in the order liquid-liquid extraction, precipitation, solid phase extraction, then magnetic bead separation:

If no options relating to purification steps are specified, then a liquid-liquid extraction followed by a precipitation will be used by default:

QuaternaryWashMagnetizationTime (1)

QuinaryWashMagnetizationTime (1)

RoboticInstrument (2)

If input cells are only Mammalian, then the bioSTAR liquid handler is used for the extraction:

If input cells have anything other than just Mammalian cells (Bacterial or Yeast), then the microbioSTAR liquid handler is used for the extraction:

SecondaryLysisIncubationInstrument (1)

If NumberOfLysisSteps is greater than 1, SecondaryLysisTime is automatically set to 15 minutes, SecondaryLysisTemperature is automatically set to Ambient, and SecondaryLysisIncubationInstrument is automatically set to an integrated instrument compatible with the specified SecondaryLysisTemperature unless otherwise specified:

SecondaryLysisMixInstrument (1)

If SecondaryLysisMixType is set to Shake, SecondaryLysisMixRate is automatically set to 200 RPM, SecondaryLysisMixTime is automatically set to 1 minute, and SecondaryLysisMixInstrument is automatically set to an available shaking device compatible with the specified SecondaryLysisMixRate and SecondaryLysisMixTemperature unless otherwise specified:

SecondaryLysisMixRate (1)

SecondaryLysisMixTemperature (1)

If SecondaryLysisMixType is set to either Pipette or Shake, SecondaryLysisMixTemperature is automatically set to Ambient unless otherwise specified:

SecondaryLysisMixTime (1)

SecondaryLysisMixType (4)

If SecondaryLysisMixVolume or SecondaryNumberOfLysisMixes are specified, SecondaryLysisMixType is automatically set to Pipette:

If SecondaryLysisMixRate, SecondaryLysisMixTime, or SecondaryLysisMixInstrument are specified, SecondaryLysisMixType is automatically set to Shake:

If NumberOfLysisSteps is greater than 1, no mixing options are set, and the sample will be mixed in a plate, SecondaryLysisMixType is automatically set to Shake:

If NumberOfLysisSteps is greater than 1, no mixing options are set, and the sample will be mixed in a tube, SecondaryLysisMixType is automatically set to Pipette:

SecondaryLysisMixVolume (2)

If SecondaryLysisMixType is set to Pipette and 50% of the total solution volume is less than 970 microliters, SecondaryLysisMixVolume is automatically set to 50% of the total solution volume:

If SecondaryLysisMixType is set to Pipette and 50% of the total solution volume is greater than 970 microliters, SecondaryLysisMixVolume is automatically set to 970 microliters:

SecondaryLysisSolution (1)

Unless otherwise specified, SecondaryLysisSolution is set to the the same solution as LysisSolution:

SecondaryLysisSolutionVolume (3)

If NumberOfLysisSteps is greater than 1 and LysisAliquot is False, SecondaryLysisSolutionVolume is automatically set to 50% of the unoccupied volume of the sample's container divided by the NumberOfLysisSteps:

If NumberOfLysisSteps is greater than 1 and LysisAliquotContainer is set, SecondaryLysisSolutionVolume is automatically set to 50% of the unoccupied volume of the LysisAliquotContainer (prior to addition of any lysis solutions) divided by the NumberOfLysisSteps:

If NumberOfLysisSteps is greater than 1, LysisAliquot is True, and LysisAliquotContainer is not set, SecondaryLysisSolutionVolume is automatically set to nine times the LysisAliquotAmount divided by the NumberOfLysisSteps:

SecondaryLysisTemperature (1)

SecondaryLysisTime (1)

SecondaryNumberOfLysisMixes (1)

If SecondaryLysisMixType is set to Pipette, SecondaryNumberOfLysisMixes is automatically set to 10:

SecondarySolidPhaseExtractionWashCentrifugeIntensity (1)

If SolidPhaseExtractionInstrument is set to a centrifuge, then SolidPhaseExtractionWashCentrifugeIntensity, SecondarySolidPhaseExtractionWashCentrifugeIntensity, and TertiarySolidPhaseExtractionWashCentrifugeIntensity are automatically set to the MaxIntensity of the Centrifuge model:

SecondarySolidPhaseExtractionWashFlowthroughContainerOut (1)

If CollectSecondarySolidPhaseExtractionWashFlowthrough is set to True, then SecondarySolidPhaseExtractionWashFlowthroughContainerOut is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate, Sterile"]:

SecondarySolidPhaseExtractionWashPressure (1)

If SolidPhaseExtractionInstrument is set to a pressure manifold, then SolidPhaseExtractionWashPressure, SecondarySolidPhaseExtractionWashPressure, and TertiarySolidPhaseExtractionWashPressure are automatically set to the MaxRoboticAirPressure:

SecondarySolidPhaseExtractionWashSolution (2)

If a SolidPhaseExtractionWashSolution is specified, then SecondarySolidPhaseExtractionWashSolution and TertiarySolidPhaseExtractionWashSolution are set to the same solution as SolidPhaseExtractionWashSolution:

SecondarySolidPhaseExtractionWashSolutionVolume (1)

If SolidPhaseExtraction is included in Purification and a SecondarySolidPhaseExtractionWashSolution is specified, then the SecondarySolidPhaseExtractionWashSolutionVolume is automatically set to 50% of the MaxVolume of the SolidPhaseExtractionCartridge:

SecondarySolidPhaseExtractionWashTime (1)

SecondaryWashMagnetizationTime (1)

SenaryWashMagnetizationTime (1)

SeptenaryWashMagnetizationTime (1)

SolidPhaseExtractionCartridgeStorageCondition (1)

SolidPhaseExtractionElutionCentrifugeIntensity (1)

If SolidPhaseExtractionInstrument is set to a centrifuge, then SolidPhaseExtractionElutionCentrifugeIntensity is automatically set to the MaxIntensity of the Centrifuge model:

SolidPhaseExtractionElutionPressure (1)

If SolidPhaseExtractionInstrument is set to a pressure manifold, then SolidPhaseExtractionElutionPressure is automatically set to the MaxRoboticAirPressure:

SolidPhaseExtractionElutionSolution (1)

SolidPhaseExtractionElutionSolutionVolume (1)

If a SolidPhaseExtractionElutionSolution is specified, then SolidPhaseExtractionElutionSolutionVolume is set to 50% of the MaxVolume of the SolidPhaseExtractionCartridge:

SolidPhaseExtractionElutionTime (1)

SolidPhaseExtractionInstrument (2)

If SolidPhaseExtractionTechnique is set to Centrifuge, SolidPhaseExtractionInstrument is automatically set to Model[Instrument, Centrifuge, "HiG4"] and SolidPhaseExtractionLoadingCentrifugeIntensity is set to the MaxRoboticCentrifugeSpeed:

If SolidPhaseExtractionTechnique is not set to Centrifuge, then SolidPhaseExtractionInstrument is set to Model[Instrument, PressureManifold, "MPE2"]:

SolidPhaseExtractionLoadingCentrifugeIntensity (1)

SolidPhaseExtractionLoadingFlowthroughContainerOut (1)

If SolidPhaseExtractionLoadingFlowthrough is set to True, then SolidPhaseExtractionLoadingFlowthroughContainerOut is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate, Sterile"]:

SolidPhaseExtractionLoadingPressure (1)

If SolidPhaseExtractionTechnique is set to Pressure, then SolidPhaseExtractionLoadingPressure is automatically set to the MaxRoboticAirPressure:

SolidPhaseExtractionLoadingSampleVolume (1)

If SolidPhaseExtraction is included in Purification and the total volume of the sample is less than the MaxVolume of the SolidPhaseExtractionCartridge, then SolidPhaseExtractionLoadingSampleVolume is automatically set to the total volume of the sample:

SolidPhaseExtractionLoadingTime (1)

SolidPhaseExtractionSeparationMode (2)

If a SolidPhaseExtractionSorbent or a SolidPhaseExtractionCartridge are specified, then SolidPhaseExtractionSeparationMode is resolved based on the SolidPhaseExtractionSorbent and the sample contents:

If neither a SolidPhaseExtractionSorbent nor a SolidPhaseExtractionCartridge are specified, then SolidPhaseExtractionSeparationMode is automatically set to IonExchange:

SolidPhaseExtractionSorbent (2)

If a SolidPhaseExtractionCartridge is specified, SolidPhaseExtractionSorbent is automatically set to the sorbent of the specified cartridge:

If a SolidPhaseExtractionCartridge is not specified, SolidPhaseExtractionSorbent is automatically set to Silica:

SolidPhaseExtractionStrategy (1)

SolidPhaseExtractionTechnique (1)

SolidPhaseExtractionWashCentrifugeIntensity (1)

SolidPhaseExtractionWashFlowthroughContainerOut (1)

If CollectSolidPhaseExtractionWashFlowthrough is set to True, then SolidPhaseExtractionWashFlowthroughContainerOut is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate, Sterile"]:

SolidPhaseExtractionWashPressure (1)

SolidPhaseExtractionWashSolution (1)

SolidPhaseExtractionWashSolutionVolume (1)

If SolidPhaseExtraction is included in Purification and a SolidPhaseExtractionWashSolution is specified, then the SolidPhaseExtractionWashSolutionVolume is automatically set to 50% of the MaxVolume of the SolidPhaseExtractionCartridge:

SolidPhaseExtractionWashTime (1)

TertiaryLysisIncubationInstrument (1)

If NumberOfLysisSteps is greater than 2, TertiaryLysisTime is automatically set to 15 minutes, TertiaryLysisTemperature is automatically set to Ambient, and TertiaryLysisIncubationInstrument is automatically set to an integrated instrument compatible with the specified TertiaryLysisTemperature unless otherwise specified:

TertiaryLysisMixInstrument (1)

If TertiaryLysisMixType is set to Shake, TertiaryLysisMixRate is automatically set to 200 RPM, TertiaryLysisMixTime is automatically set to 1 minute, and TertiaryLysisMixInstrument is automatically set to an available shaking device compatible with the specified TertiaryLysisMixRate and TertiaryLysisMixTemperature unless otherwise specified:

TertiaryLysisMixRate (1)

TertiaryLysisMixTemperature (1)

If TertiaryLysisMixType is set to either Pipette or Shake, TertiaryLysisMixTemperature is automatically set to Ambient unless otherwise specified:

TertiaryLysisMixTime (1)

TertiaryLysisMixType (4)

If TertiaryLysisMixVolume or TertiaryNumberOfLysisMixes are specified, TertiaryLysisMixType is automatically set to Pipette:

If TertiaryLysisMixRate, TertiaryLysisMixTime, or TertiaryLysisMixInstrument are specified, TertiaryLysisMixType is automatically set to Shake:

If NumberOfLysisSteps is greater than 2, no mixing options are set, and the sample will be mixed in a plate, TertiaryLysisMixType is automatically set to Shake:

If NumberOfLysisSteps is greater than 2, no mixing options are set, and the sample will be mixed in a tube, TertiaryLysisMixType is automatically set to Pipette:

TertiaryLysisMixVolume (2)

If TertiaryLysisMixType is set to Pipette and 50% of the total solution volume is less than 970 microliters, TertiaryLysisMixVolume is automatically set to 50% of the total solution volume:

If TertiaryLysisMixType is set to Pipette and 50% of the total solution volume is greater than 970 microliters, TertiaryLysisMixVolume is automatically set to 970 microliters:

TertiaryLysisSolution (1)

If NumberOfLysisSteps is greater than 2, TertiaryLysisSolution is the same as LysisSolution unless otherwise specified:

TertiaryLysisSolutionVolume (3)

If NumberOfLysisSteps is greater than 2 and LysisAliquot is False, TertiaryLysisSolutionVolume is automatically set to 50% of the unoccupied volume of the sample's container divided by the NumberOfLysisSteps:

If NumberOfLysisSteps is greater than 2 and LysisAliquotContainer is set, TertiaryLysisSolutionVolume is automatically set to 50% of the unoccupied volume of the LysisAliquotContainer (prior to addition of the TertiaryLysisSolutionVolume) divided by the NumberOfLysisSteps:

If NumberOfLysisSteps is greater than 2, LysisAliquot is True, and LysisAliquotContainer is not set, TertiaryLysisSolutionVolume is automatically set to nine times the LysisAliquotAmount divided by the NumberOfLysisSteps:

TertiaryLysisTemperature (1)

TertiaryLysisTime (1)

TertiaryNumberOfLysisMixes (1)

If TertiaryLysisMixType is set to Pipette, TertiaryNumberOfLysisMixes is automatically set to 10:

TertiarySolidPhaseExtractionWashCentrifugeIntensity (1)

TertiarySolidPhaseExtractionWashFlowthroughContainerOut (1)

If CollectTertiarySolidPhaseExtractionWashFlowthrough is set to True, then TertiarySolidPhaseExtractionWashFlowthroughContainerOut is automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate, Sterile"]:

TertiarySolidPhaseExtractionWashPressure (1)

TertiarySolidPhaseExtractionWashSolution (2)

TertiarySolidPhaseExtractionWashSolutionVolume (1)

If SolidPhaseExtraction is included in Purification and a TertiarySolidPhaseExtractionWashSolution is specified, then the TertiarySolidPhaseExtractionWashSolutionVolume is automatically set to 50% of the MaxVolume of the SolidPhaseExtractionCartridge:

TertiarySolidPhaseExtractionWashTime (1)

TertiaryWashMagnetizationTime (1)

UnprecipitatedSampleContainerLabel (1)

UnprecipitatedSampleContainerOut (1)

If PrecipitationTargetPhase is set to Liquid, then UnprecipitatedSampleContainerOut is automatically set to a container selected by PreferredContainer[...] based on having sufficient capacity to not overflow when the separated liquid phase is added.

UnprecipitatedSampleLabel (1)

UnprecipitatedSampleStorageCondition (1)

WashMagnetizationTime (1)

WorkCell (2)

If input cells are only Mammalian, then the bioSTAR WorkCell is used for the extraction:

If input cells have anything other than just Mammalian cells (Bacterial or Yeast), then the microbioSTAR WorkCell is used for the extraction:

Messages (16)

AqueousSolventOptionsMismatched (1)

An error is returned if there is a mix of specified and unspecified aqueous solvent options (AqueousSolvent, AqueousSolventVolume, AqueousSolventRatio) for a sample:

DemulsifierOptionsMismatched (1)

An error is returned if there is a mix of specified and unspecified demulsifier options (Demulsifier, DemulsifierAmount, DemulsifierAdditions):

Error::ConflictingLysisOutputOptions (1)

An error is returned if the last step of the extraction is lysis, but the ouput options do not match their counterparts in the lysis options:

Error::InvalidExtractionMethod (1)

An error is returned if a plasmid DNA extraction method is specified and it does not pass ValidObjectQ:

Error::MethodOptionConflict (1)

An error is returned if a plasmid DNA extraction method is specified but an option is set differently from the method-specified value:

InvalidSolidMediaSample (1)

An error is returned if the input cell samples are in solid media since only suspended or adherent cells are supported for plasmid DNA extraction:

LiquidLiquidExtractionOptionMismatch (1)

An error is returned if any liquid-liquid extraction options are set, but LiquidLiquidExtraction is not specified in Purification:

LiquidLiquidExtractionSelectionStrategyOptionsMismatched (1)

A warning is returned if a selection strategy (LiquidLiquidExtractionSelectionStrategy) is specified, but there is only one liquid-liquid extraction round specified (NumberOfLiquidLiquidExtractions) since a selection strategy is only required for multiple rounds of liquid-liquid extraction:

LiquidLiquidExtractionStepCountLimitExceeded (1)

An error is returned if there are more than 3 liquid-liquid extractions called for in the purification step list:

MagneticBeadSeparationStepCountLimitExceeded (1)

An error is returned if there are more than 3 magnetic bead separations called for in the purification step list:

NeutralizeOptionMismatch (1)

An error is returned if neutralization options are set if Neutralize is False or vice versa:

NoPlasmidDNAExtractionStepsSet (1)

An error is returned if no steps are selected for an extraction (neither lysis, neutralization, nor any purification techniques):

OrganicSolventOptionsMismatched (1)

An error is returned if there is a mix of specified and unspecified organic solvent options (OrganicSolvent, OrganicSolventVolume, OrganicSolventRatio) for a sample:

PurificationStepCountLimitExceeded (1)

An error is returned if there are more than 3 precipitations called for in the purification step list:

SolidPhaseExtractionStepCountLimitExceeded (1)

An error is returned if there are more than 3 solid phase extractions called for in the purification step list:

SolidPhaseExtractionTechniqueInstrumentMismatch (1)

Return an error if the specified SolidPhaseExtractionInstrument cannot perform the specified SolidPhaseExtractionTechnique (If MPE2 is selected, the Technique must be Pressure. If HiG4 is selected, the Technique must be Centrifuge):

Last modified on Wed 26 Jun 2024 14:48:56